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Magnetic particles–based biosensor for biogenic amines using an optical oxygen sensor as a transducer

SYSNO ASEP	0388384
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Magnetic particles–based biosensor for biogenic amines using an optical oxygen sensor as a transducer
Author(s)	Pospišková, K. (CZ) Šafařík, Ivo (UEK-B)_{RID, SAI} Šebela, M. (CZ) Kuncová, Gabriela (UCHP-M)_{RID, SAI}
Number of authors	4
Source Title	Microchimica Acta. - : Springer - ISSN 0026-3672 Roč. 180, 3-4 (2013), s. 311-318
Number of pages	8 s.
Language	eng - English
Country	AT - Austria
Keywords	magnetic carriers ; optical fibre biosensor ; diamine oxidase ; fluorescence quenching ; ruthenium complex
Subject RIV	EH - Ecology, Behaviour
Subject RIV - cooperation	Institute of Chemical Process Fundamentals - Biochemistry
R&D Projects	IAAX08240901 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
Institutional support	RVO:67179843 - RVO:67179843 ; UCHP-M - RVO:67985858
UT WOS	000313837900017
DOI	10.1007/s00604-012-0932-0
Annotation	We have developed a fibre optic biosensor with incorporated magnetic microparticles for the determination of biogenic amines. The enzyme diamine oxidase from Pisum sativum was immobilized either on chitosan-coated magnetic microparticles or on commercial microbeads modified with a ferrofluid. Both the immobilized enzyme and the ruthenium complex were incorporated into a UV-cured inorganic–organic polymer composite and deposited on a lens that was connected, by optical fibres, to an electro-optical detector. The enzyme catalyzes the oxidation of amines under consumption of oxygen. The latter was determined by measuring the quenched fluorescence lifetime of the ruthenium complex. The limits of detection for the biogenic amines putrescine and cadaverine are 25–30 μmol L−1, and responses are linear up to a concentration of 1 mmol L−1.
Workplace	Global Change Research Institute
Contact	Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268
Year of Publishing	2013

Number of the records: 1