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Auxin binding proteins ABP1 and ABP4 are involved in the light- and auxin-induced down-regulation of phytochrome gene PHYB in maize (Zea mays L.) mesocotyl
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SYSNO ASEP 0385358 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Auxin binding proteins ABP1 and ABP4 are involved in the light- and auxin-induced down-regulation of phytochrome gene PHYB in maize (Zea mays L.) mesocotyl Author(s) Bořucká, Jana (UEB-Q)
Fellner, Martin (UEB-Q) RID, ORCIDSource Title Plant Growth Regulation. - : Springer - ISSN 0167-6903
Roč. 68, č. 3 (2012), s. 503-509Number of pages 7 s. Language eng - English Country NL - Netherlands Keywords Auxin binding protein ; ABP1 ; ABP4 Subject RIV EF - Botanics R&D Projects 1P05ME792 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000310969900018 DOI 10.1007/s10725-012-9719-x Annotation Previous research in maize suggested a possible involvement of auxin-binding proteins (ABPs) in light signaling during maize seedling development. To obtain more information about the interaction of auxin and light signaling, we investigated the gene expression of phytochrome B (PHYB) and phytochrome A (PHYA) in loss-of-function mutants in ABP1 and ABP4 genes in maize (Zea mays L.). We studied how expression of the PHYB and PHYA genes in mesocotyl is regulated by white light (WL), and whether exogenous auxin NAA influences the expression of the phytochrome genes. We found that knockout of ABP1 or ABP4 results in essentially reduced expression of PHYB gene in dark-grown mesocotyl. WL reduced PHYB expression in WT but not in the ABPs knockout seedlings. The data indicate that ABP1 and ABP4 are positively involved in PHYB expression in etiolated mesocotyl. Our results also indicate that in etiolated mesocotyl, ABP1 and ABP4 mediate the inhibitory effect of exogenous auxin on level of PHYB transcript. In contrast, in our experimental conditions, WL does not reduce expression of PHYA. Our results further suggest that ABP1 and ABP4 are not likely involved in the expression of PHYA gene and neither in auxin-induced suppression of PHYA transcript accumulation. Our results support the existence of cross-talk between auxin and light signaling and indicate for the first time that ABP1, ABP4 and PHYB genes could share common signaling pathway(s). Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2013
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