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Tissue-specific profiling of the Arabidopsis thaliana auxin metabolome
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SYSNO ASEP 0384241 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Tissue-specific profiling of the Arabidopsis thaliana auxin metabolome Author(s) Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Hényková, Eva (UEB-Q) ORCID
Sairanen, I. (SE)
Kowalczyk, M. (SE)
Pospíšil, Tomáš (UEB-Q) ORCID
Ljung, K. (SE)Source Title Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 72, č. 3 (2012), s. 523-536Number of pages 14 s. Language eng - English Country GB - United Kingdom Keywords indole-3-acetic acid ; auxin ; biosynthesis Subject RIV EC - Immunology R&D Projects KAN200380801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000310343200015 DOI 10.1111/j.1365-313X.2012.05085.x Annotation The plant hormone auxin is believed to influence almost every aspect of plant growth and development. Auxin transport, biosynthesis and degradation combine to form gradients of the hormone that influence a range of key developmental and environmental response processes. There is abundant genetic evidence for the existence of multiple pathways for auxin biosynthesis and degradation. The complexity of these pathways makes it difficult to obtain a clear picture of the relative importance of specific metabolic pathways during development. We have developed a sensitive mass spectrometry-based method to simultaneously profile the majority of known auxin precursors and conjugates/catabolites in small amounts of Arabidopsis tissue. The method includes a new derivatization technique for quantification of the most labile of the auxin precursors. We validated the method by profiling the auxin metabolome in root and shoot tissues from various Arabidopsis thaliana ecotypes and auxin over-producing mutant lines. Substantial differences were shown in metabolite patterns between the lines and tissues. We also found differences of several orders of magnitude in the abundance of auxin metabolites, potentially indicating the relative importance of these compounds in the maintenance of auxin levels and activity. The method that we have established will enable researchers to obtain a better understanding of the dynamics of auxin metabolism and activity during plant growth and development. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2013
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