Number of the records: 1

Interallelic and intergenic incompatibilities of the Prdm9 (Hst1) gene in mouse hybrid sterility

SYSNO ASEP	0382929
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Interallelic and intergenic incompatibilities of the Prdm9 (Hst1) gene in mouse hybrid sterility
Author(s)	Flachs, Petr (UMG-J) Mihola, Ondřej (UMG-J)_{RID, ORCID} Šimeček, Petr (UMG-J) Gregorová, Soňa (UMG-J) Schimenti, J.C. (US) Matsui, Y. (JP) Baudat, F. (FR) de Massy, B. (FR) Piálek, Jaroslav (UBO-W)_{RID, ORCID, SAI} Forejt, Jiří (UMG-J)_{RID, ORCID} Trachtulec, Zdeněk (UMG-J)_{RID, ORCID}
Source Title	PLoS Genetics. - : Public Library of Science - ISSN 1553-7404 Roč. 8, č. 11 (2012), e1003044
Number of pages	10 s.
Publication form	Online - E
Language	eng - English
Country	US - United States
Keywords	hybrid sterility ; meiosis ; incompatibility
Subject RIV	EB - Genetics ; Molecular Biology
Subject RIV - cooperation	Institute of Molecular Genetics
R&D Projects	1M0520 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GAP305/10/1931 GA ČR - Czech Science Foundation (CSF)
	GA206/08/0640 GA ČR - Czech Science Foundation (CSF)
	GPP305/11/P630 GA ČR - Czech Science Foundation (CSF)
Institutional support	UBO-W - RVO:68081766 ; UMG-J - RVO:68378050
CEZ	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000311891600032
EID SCOPUS	84870708407
DOI	10.1371/journal.pgen.1003044
Annotation	The Dobzhansky-Muller model of incompatibilities explains reproductive isolation between species by incorrect epistatic interactions. Although the mechanisms of speciation are of great interest, no incompatibility has been characterized at the gene level in mammals. The Hybrid sterility 1 gene (Hst1) participates in the arrest of meiosis in F(1) males of certain strains from two Mus musculus subspecies, e.g., PWD from M. m. musculus and C57BL/6J (henceforth B6) from M. m. domesticus. Hst1 has been identified as a meiotic PR-domain gene (Prdm9) encoding histone 3 methyltransferase in the male offspring of PWD females and B6 males, (PWD×B6)F(1). To characterize the incompatibilities underlying hybrid sterility, we phenotyped reproductive and meiotic markers in males with altered copy numbers of Prdm9. A partial rescue of fertility was observed upon removal of the B6 allele of Prdm9 from the azoospermic (PWD×B6)F(1) hybrids, whereas removing one of the two Prdm9 copies in PWD or B6 background had no effect on male reproduction. Incompatibility(ies) not involving Prdm9(B6) also acts in the (PWD×B6)F(1) hybrids, since the correction of hybrid sterility by Prdm9(B6) deletion was not complete. Additions and subtractions of Prdm9 copies, as well as allelic replacements, improved meiotic progression and fecundity also in the progeny-producing reciprocal (B6×PWD)F(1) males. Moreover, an increased dosage of Prdm9 and reciprocal cross enhanced fertility of other sperm-carrying male hybrids, (PWD×B6-C3H.Prdm9)F(1), harboring another Prdm9 allele of M. m. domesticus origin. The levels of Prdm9 mRNA isoforms were similar in the prepubertal testes of all types of F(1) hybrids of PWD with B6 and B6-C3H.Prdm9 despite their different prospective fertility, but decreased to 53% after removal of Prdm9(B6). Therefore, the Prdm9(B6) allele probably takes part in posttranscriptional dominant-negative hybrid interaction(s) absent in the parental strains.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2015

Number of the records: 1