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Regulation of tissue differentiation by plant growth regulators on tTCLs of Panax ginseng adventitious roots

SYSNO ASEP	0382535
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Regulation of tissue differentiation by plant growth regulators on tTCLs of Panax ginseng adventitious roots
Author(s)	Langhansová, Lenka (UEB-Q)_{RID, ORCID} Maršík, Petr (UEB-Q)_{RID, ORCID} Vaněk, Tomáš (UEB-Q)_{RID, ORCID}
Source Title	Industrial Crops and Products. - : Elsevier - ISSN 0926-6690 Roč. 35, č. 1 (2012), s. 154-159
Number of pages	6 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Adventitious roots ; Panax ginseng ; Ginsenosides
Subject RIV	CE - Biochemistry
R&D Projects	KJB400550705 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	ME08070 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000296110700021
DOI	10.1016/j.indcrop.2011.06.028
Annotation	Differentiated tissue in Panax ginseng cultures was found to be very efficacious for saponin production. In order to increase the yield of saponins and preserve culture stability we were testing different plant growth regulators (PGR) and auxin/cytokinin combinations to regulate a level of tissue differentiation. For this purpose we used transverse thin cell layers (tTCLs) of adventitious roots of Panax ginseng. Adventitious roots were cultivated in Shenk and Hildebrand (SH) liquid medium supplemented with IBA (24.6 mu M). Callus formation and root multiplication of adventitious root tTCLs was evaluated after 4 and following 12 weeks of cultivation, respectively, on SH basal medium containing various auxins (3 mg l(-1)) or cytokinins (0.2 or 0.02 mg l(-1)) or their combinations. We found that kinetin (Kin) in combination with auxin benzo[b]selenienyl acetic acid (BSAA), naphthalene acetic acid or indole-3-butric acidis the best for biomass production and following root multiplication. These combinations were tested in previously selected most suitable large-scale system a temporary immersion system RITA. The best saponin production (15.94 +/- 1.89 mg g(-1) dry weight) and growth value (5.62 +/- 0.34) was reached on medium containing BSAA and Kin combination.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2013

Number of the records: 1