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beta-aminobutyric acid protects Brassica napus plants from infection by Leptosphaeria maculans. Resistance induction or a direct antifungal effect?
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SYSNO ASEP 0382522 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title beta-aminobutyric acid protects Brassica napus plants from infection by Leptosphaeria maculans. Resistance induction or a direct antifungal effect? Author(s) Šašek, Vladimír (UEB-Q) RID
Nováková, Miroslava (UEB-Q) RID
Dobrev, Petre (UEB-Q) RID, ORCID
Valentová, O. (CZ)
Burketová, Lenka (UEB-Q) RID, ORCIDSource Title European journal of plant pathology. - : Springer - ISSN 0929-1873
Roč. 133, č. 1 (2012), s. 279-289Number of pages 11 s. Language eng - English Country NL - Netherlands Keywords Antimicrobial ; BTH ; Gene expression Subject RIV GF - Plant Pathology, Vermin, Weed, Plant Protection R&D Projects GA522/08/1581 GA ČR - Czech Science Foundation (CSF) QH72117 GA MZe - Ministry of Agriculture (MZe) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000302232600019 DOI 10.1007/s10658-011-9897-9 Annotation Resistance to infection in plants can be induced by treatment with various chemicals. One such compound is beta-aminobutyric acid (BABA). Its positive effect on disease resistance has been noted in several pathosystems. Here we demonstrate that treatment with BABA protects Brassica napus plants from infection by the fungal pathogen Leptosphaeria maculans. Surprisingly, BABA also displayes in vitro antifungal activity against L. maculans with EC50 similar to the fungicide tebuconazole. Both spore germination and hyphal growth were affected. The toxic effect can be reverted by addition of trypton to the culture medium. We hypothesised that BABA might inhibit inorganic nitrogen assimilation. Suppression of disease progression in plants and antifungal activity in vitro was weaker for alpha-aminobutyric acid and negligible for gamma-aminobutyric acid. In contrast to a resistance inducer benzothiadiazole, the effect of BABA on disease development was nearly independent of the timing of treatment, indicating possible antifungal activity in planta. On the other hand, quantification of multiple hormones and an expression analysis have shown that treatment with BABA induces a synthesis of salicylic acid (SA) and expression of SA marker gene PR-1, but no evidence was observed for priming of SA responses to L. maculans. While we have not conclusively demonstrated how BABA suppresses the disease progression, our results do indicate that antifungal activity is another mechanism by which BABA can protect plants from infection. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2013
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