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Expression of GFP-mTalin reveals an actin-related role for the Arabidopsis Class II formin AtFH12

SYSNO ASEP	0382131
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Expression of GFP-mTalin reveals an actin-related role for the Arabidopsis Class II formin AtFH12
Author(s)	Cvrčková, F. (CZ) Grunt, M. (CZ) Žárský, Viktor (UEB-Q)_{RID, ORCID}
Source Title	Biologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134 Roč. 56, č. 3 (2012), s. 431-440
Number of pages	10 s.
Language	eng - English
Country	NL - Netherlands
Keywords	FH2 proteins ; genetic redundancy ; salt stress
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GAP305/10/0433 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000303062400005
DOI	10.1007/s10535-012-0071-9
Annotation	Formins (FH2 proteins) are implicated in F-actin nucleation and other aspects of cytoskeletal organization. Plants possess two formin clades, relatively well-described Class I formins and so far poorly characterized Class II formins. Comparison of Class II formin genes of two Arabidopsis species, A. thaliana and A. lyrata, indicates dynamic evolution within the Class II formin clade. Disruption of an outlier A. thaliana Class II formin gene, AtFH12 (At1g42980), whose expression is induced by NaCl, produced only negligible phenotypic effects under a variety of conditions, including salt stress, suggesting functional redundancy among Class II formins. However, the same mutation massively aggravated toxic effects of the expression of a fluorescent actin marker, GFP-tagged mouse talin (GFP-mTalin), known to interfere with normal actin dynamics. Abnormal actin structures were observed in atfh12 mutants expressing GFP-mTalin as compared to wild type. This not only demonstrates an actin-associated function for AtFH12, but also documents the feasibility of using the heterologous actin marker to "stress-test" the actin cytoskeleton in phenotyping "weak" actin related mutant alleles.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2013

Number of the records: 1