Number of the records: 1
Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants
- 1.
SYSNO ASEP 0380685 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants Author(s) Čeřovská, Noemi (UEB-Q) RID, ORCID
Hoffmeisterová, Hana (UEB-Q) RID
Moravec, Tomáš (UEB-Q) RID, ORCID
Plchová, Helena (UEB-Q) RID, ORCID
Folwarczna, Jitka (UEB-Q)
Synková, Helena (UEB-Q) RID, ORCID
Ryšlavá, H. (CZ)
Ludvíková, V. (CZ)
Šmahel, M. (CZ)Source Title Journal of Biosciences. - : Indian Academy of Sciences - ISSN 0250-5991
Roč. 37, č. 1 (2012), s. 125-133Number of pages 9 s. Language eng - English Country IN - India Keywords Human papillomavirus (HPV-16) ; L2-and E7-derived epitopes ; transient expression Subject RIV FD - Oncology ; Hematology R&D Projects GA521/06/0973 GA ČR - Czech Science Foundation (CSF) GA521/09/1525 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000301163400015 DOI 10.1007/s12038-011-9177-z Annotation Transient expression of foreign genes based on plant viral vectors is a suitable system for the production of immunogens that can be used for development of a new generation of vaccines against a variety of infectious diseases. In this study the epitope derived from HPV-16 L2 minor capsid protein (amino acids 108–120) was expressed from Potato virus X (PVX)-based vector pGR106 as N- or C-terminal fusion with the PVX coat protein (PVX CP) in transgenic Nicotiana benthamiana plants. The protein L2108-120-PVX CP was expressed in plants at a level of 170 mg/kg of fresh leaf tissue. The protein PVX CP-L2108-120 was expressed using mutated vector to avoid homologous recombination at a level of 8 mg/kg of fresh leaf tissue. Immunogenicity of L2108-120-PVX CP virus-like particles was tested after immunization of mice by subcutaneous injection or tattoo administration. In animal sera the antibodies against the PVX CP and the L2108-120 epitope were found after both methods of vaccine delivery. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2013
Number of the records: 1