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Next-generation sequencing and syntenic integration of flow-sorted arms of wheat chromosome 4A exposes the chromosome structure and gene content
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SYSNO ASEP 0380583 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Next-generation sequencing and syntenic integration of flow-sorted arms of wheat chromosome 4A exposes the chromosome structure and gene content Author(s) Hernandez, P. (ES)
Martis, M. (DE)
Dorado, G. (ES)
Pfeifer, M. (DE)
Galvez, S. (ES)
Schaaf, S. (DE)
Jouve, N. (ES)
Šimková, Hana (UEB-Q) RID, ORCID
Valárik, Miroslav (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCID
Mayer, K. F. X. (DE)Source Title Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 69, č. 3 (2012), s. 377-386Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords wheat genome ; chromosome sorting ; genome zipper Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA521/08/1629 GA ČR - Czech Science Foundation (CSF) GAP501/10/1740 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000299553100001 DOI 10.1111/j.1365-313X.2011.04808.x Annotation Wheat is the third most important crop for human nutrition in the world. The availability of high-resolution genetic and physical maps and ultimately a complete genome sequence holds great promise for breeding improved varieties to cope with increasing food demand under the conditions of changing global climate. However, the large size of the bread wheat (Triticum aestivum) genome (approximately 17 Gb/1C) and the triplication of genic sequence resulting from its hexaploid status have impeded genome sequencing of this important crop species. Here we describe the use of mitotic chromosome flow sorting to separately purify and then shotgun-sequence a pair of telocentric chromosomes that together form chromosome 4A (856 Mb/1C) of wheat. The isolation of this much reduced template and the consequent avoidance of the problem of sequence duplication, in conjunction with synteny-based comparisons with other grass genomes, have facilitated construction of an ordered gene map of chromosome 4A, embracing >= 85% of its total gene content, and have enabled precise localization of the various translocation and inversion breakpoints on chromosome 4A that differentiate it from its progenitor chromosome in the A genome diploid donor. The gene map of chromosome 4A, together with the emerging sequences of homoeologous wheat chromosome groups 4, 5 and 7, represent unique resources that will allow us to obtain new insights into the evolutionary dynamics between homoeologous chromosomes and syntenic chromosomal regions. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2013
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