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ER disruption and GFP degradation during non-regenerable transformation of flax with Agrobacterium tumefaciens
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SYSNO ASEP 0379091 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title ER disruption and GFP degradation during non-regenerable transformation of flax with Agrobacterium tumefaciens Author(s) Bleho, J. (SK)
Obert, B. (SK)
Takáč, T. (CZ)
Petrovská, Beáta (UEB-Q) RID, ORCID
Heym, C. (DE)
Menzel, D. (DE)
Šamaj, J. (CZ)Source Title Protoplasma - ISSN 0033-183X
Roč. 249, č. 1 (2012), s. 53-63Number of pages 11 s. Language eng - English Country AT - Austria Keywords Agrobacterium rhizogenes ; Agrobacterium tumefaciens ; Endoplasmic reticulum Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000298754000006 DOI 10.1007/s00709-010-0261-2 Annotation Flax is considered as plant species susceptible to Agrobacterium-mediated genetic transformation. In this study, stability of flax transformation by Agrobacterium rhizogenes versus Agrobacterium tumefaciens was tested by using combined selection for antibiotic resistance and visual selection of green fluorescent protein (GFP)-fusion reporter targeted to the endoplasmic reticulum (ER). Transformation with A. rhizogenes was stable for over 2 years, whereas transformation by A. tumefaciens resulted in non-regenerable stable transformation which was restricted solely to transgenic callus and lasted only 6-8 weeks. However, shoots regenerated from this callus appeared to be non-transgenic. Importantly, callus and root cells stably transformed with A. rhizogenes showed typical regular organization and dynamics of ER as visualized by GFP-ER marker. On the other hand, callus cells transformed with A. tumefaciens showed disintegrated ER structure and impaired dynamics which was accompanied with developmental degradation of GFP. Consequently, shoots which regenerated from such callus were all non-transgenic. Possible reasons for this non-regenerable flax transformation by A. tumefaciens are discussed. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2013
Number of the records: 1