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Expression of Human papillomavirus 16 E7ggg oncoprotein on N- and C-terminus of Potato virus X coat protein in bacterial and plant cells

SYSNO ASEP	0364095
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Expression of Human papillomavirus 16 E7ggg oncoprotein on N- and C-terminus of Potato virus X coat protein in bacterial and plant cells
Author(s)	Plchová, Helena (UEB-Q)_{RID, ORCID} Moravec, Tomáš (UEB-Q)_{RID, ORCID} Hoffmeisterová, Hana (UEB-Q)_RID Folwarczna, Jitka (UEB-Q) Čeřovská, Noemi (UEB-Q)_{RID, ORCID}
Source Title	Protein Expression and Purification. - : Elsevier - ISSN 1046-5928 Roč. 77, č. 2 (2011), s. 146-152
Number of pages	7 s.
Language	eng - English
Country	US - United States
Keywords	Bacterial expression ; Human papillomavirus ; Oncoprotein E7
Subject RIV	EI - Biotechnology ; Bionics
R&D Projects	GA521/09/1525 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000288591900003
DOI	10.1016/j.pep.2011.01.008
Annotation	The E7 oncoprotein from Human papillomavirus type 16 (HPV16) is an attractive candidate for anti-cancer therapeutical vaccine development. In this study, we engineered different fusions of mutagenized coding sequence of E7 oncoprotein (E7ggg) with coat protein of Potato virus X (PVX CP) both on 5`- and 3`-terminus of PVX CP and evaluated the influence of the length of linker (no linker, 4, 15 aa) connecting PVX CP and E7ggg on their production. At first the expression in Escherichia coil was conducted to assess the characteristics of the recombinant protein prior to be further produced in plants, that is, resultant proteins were used for screening of their immunological reactivity with antibodies against PVX CP and E7. Fusion proteins successfully expressed in bacteria and plants were partially purified and their reactivity and ability to form virus-like particles were evaluated with anti-E7 antibodies.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2012

Number of the records: 1