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The plant formin AtFH4 interacts with both actin and microtubules, and contains a newly identified microtubule-binding domain
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SYSNO ASEP 0350394 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The plant formin AtFH4 interacts with both actin and microtubules, and contains a newly identified microtubule-binding domain Author(s) Deeks, M.J. (GB)
Fendrych, Matyáš (UEB-Q)
Smertenko, A. (GB)
Bell, K.S. (GB)
Oparka, K. (GB)
Cvrčková, F. (CZ)
Žárský, Viktor (UEB-Q) RID, ORCID
Hussey, P.J. (GB)Source Title Journal of Cell Science. - : Company of Biologists - ISSN 0021-9533
Roč. 123, č. 8 (2010), s. 1209-1215Number of pages 7 s. Language eng - English Country GB - United Kingdom Keywords Actin regulating proteins ; Membrane ; Microtubule Subject RIV EB - Genetics ; Molecular Biology R&D Projects LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP305/10/0433 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000276568200004 DOI 10.1242/jcs.065557 Annotation The dynamic behaviour of the actin cytoskeleton in plants relies on the coordinated action of several classes of actin-binding proteins (ABPs). These ABPs include the plant-specific subfamilies of actin-nucleating formin proteins. The model plant species Arabidopsis thaliana has over 20 formin proteins, all of which contain plant-specific regions in place of the GTPase-binding domain, formin homology (FH)3 domain, and DAD and DID motifs found in many fungal and animal formins. We have identified for the first time a plant-specific region of the membrane-integrated formin AtFH4 that mediates an association with the microtubule cytoskeleton. In vitro analysis shows that this region (named the GOE domain) binds directly to microtubules. Overexpressed AtFH4 accumulates at the endoplasmic reticulum membrane and co-aligns the endoplasmic reticulum with microtubules. The FH1 and FH2 domains of formins are conserved in plants, and we show that these domains of AtFH4 nucleate F-actin. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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