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Mutual regulation of plant phospholipase D and the actin cytoskeleton
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SYSNO ASEP 0350391 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Mutual regulation of plant phospholipase D and the actin cytoskeleton Author(s) Pleskot, Roman (UEB-Q) RID, ORCID
Potocký, Martin (UEB-Q) RID, ORCID
Pejchar, Přemysl (UEB-Q) RID, ORCID
Linek, J. (CZ)
Bezvoda, R. (CZ)
Martinec, Jan (UEB-Q) RID, ORCID
Valentová, O. (CZ)
Novotná, Z. (CZ)
Žárský, Viktor (UEB-Q) RID, ORCIDSource Title Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 62, č. 3 (2010), s. 494-507Number of pages 14 s. Language eng - English Country GB - United Kingdom Keywords phospholipase D ; actin ; signaling Subject RIV ED - Physiology R&D Projects IAA601110916 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) LC06034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA522/05/0340 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000276659400013 DOI 10.1111/j.1365-313X.2010.04168.x Annotation Membrane lipids and cytoskeleton dynamics are intimately inter-connected in the eukaryotic cell and phospholipase D (PLD) and its product phosphatidic acid (PA) were discovered to be important regulators in the membrane–cytoskeleton interface in eukaryotes. Here we report the mechanistic details of plant PLD–actin interactions. Inhibition of PLD by compromises, and PA rescues, pollen tube actin. We identified NtPLDb1 as a regulatory partner of actin, by both activity and in vitro interaction assays. The activity of tobacco PIP2-dependent PLD (PLDb) is specifically enhanced by F-actin and inhibited by G-actin. Using sequence- and structure-based analysis, together with site-directed mutagenesis, we identified Asn323 and Thr382 of NtPLDb1 as the crucial amino acids in the actin-interacting fold. The effect of antisense-mediated suppression of NtPLDb1 or NtPLDd on pollen tube F-actin dynamics shows that NtPLDb1 is the active partner in PLD–actin interplay. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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