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Aluminium ions inhibit the formation of diacylglycerol generated by phosphatidylcholine-hydrolysing phospholipase C in tobacco cells

SYSNO ASEP	0349924
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Aluminium ions inhibit the formation of diacylglycerol generated by phosphatidylcholine-hydrolysing phospholipase C in tobacco cells
Author(s)	Pejchar, Přemysl (UEB-Q)_{RID, ORCID} Potocký, Martin (UEB-Q)_{RID, ORCID} Novotná, Z. (CZ) Veselková, Štěpánka (UEB-Q) Kocourková, Daniela (UEB-Q)_ORCID Valentová, O. (CZ) Schwarzerová, K. (CZ) Martinec, Jan (UEB-Q)_{RID, ORCID}
Source Title	New Phytologist - ISSN 0028-646X Roč. 188, č. 1 (2010), s. 150-160
Number of pages	11 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	aluminium (AL) ; BY-2 ; diacylglycerol (DAG)
Subject RIV	ED - Physiology
R&D Projects	GA522/07/1614 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000281551500017
DOI	10.1111/j.1469-8137.2010.03349.x
Annotation	Aluminium ions (Al) have been recognized as a major toxic factor for crop production in acidic soils. This study aimed to assess the impact of Al on the activity of phosphatidylcholine-hydrolysing phospholipase C (PC-PLC), a new member of the plant phospholipase family.We labelled the tobacco cell line BY-2 and pollen tubes with a fluorescent derivative of phosphatidylcholine and assayed for patterns of fluorescently labelled products. Growth of pollen tubes was analysed.We observed a significant decrease of labelled diacylglycerol (DAG) in cells treated with AlCl3. Investigation of possible metabolic pathways that control DAG generation and consumption during the response to Al showed that DAG originated from the reaction catalysed by PC-PLC. The growth of pollen tubes was retarded in the presence of Al and this effect was accompanied by the decrease of labelled DAG similar to the case of the BY-2 cell line. The growth of pollen tubes arrested by Al was rescued by externally added DAG.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2011

Number of the records: 1