Cytokinin receptor antagonists derived from 6-benzylaminopurine

Nisler, Jaroslav; Zatloukal, Marek; Popa, Igor; Doležal, Karel; Strnad, Miroslav; Spíchal, Lukáš

doi:https://dx.doi.org/10.1016/j.phytochem.2010.01.018

Number of the records: 1

Cytokinin receptor antagonists derived from 6-benzylaminopurine

1.

SYSNO ASEP	0348001
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Cytokinin receptor antagonists derived from 6-benzylaminopurine
Author(s)	Nisler, Jaroslav (UEB-Q)_{RID, ORCID} Zatloukal, Marek (UEB-Q)_ORCID Popa, Igor (UEB-Q) Doležal, Karel (UEB-Q)_{RID, ORCID} Strnad, Miroslav (UEB-Q)_{RID, ORCID} Spíchal, Lukáš (UEB-Q)_{RID, ORCID}
Source Title	Phytochemistry. - : Elsevier - ISSN 0031-9422 Roč. 71, č. 7 (2010), s. 823-830
Number of pages	8 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Cytokinin ; Anticytokinin ; Cytokinin receptor
Subject RIV	CE - Biochemistry
R&D Projects	GD522/08/H003 GA ČR - Czech Science Foundation (CSF)
	GP522/07/P197 GA ČR - Czech Science Foundation (CSF)
	LC06034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000277908400016
DOI	10.1016/j.phytochem.2010.01.018
Annotation	Recently we reported 6-(2-hydroxy-3-methylbenzylamino)purine (PI-55) as the first molecule to antagonize cytokinin activity at the receptor level. Here we report the synthesis and in vitro biological testing of eleven BAP derivatives substituted in the benzyl ring and in the C2, N7 and N9 positions of the purine moiety. The ability of the compounds to interact with Arabidopsis cytokinin receptors AHK3 and CRE1/AHK4 was tested in bacterial receptor and in live-cell binding assays, and in an Arabidopsis ARR5:GUS (Arabidopsis response regulator 5) reporter gene assay. Cytokinin activity of the compounds was determined in classical cytokinin biotests (tobacco callus, wheat leaf senescence and Amaranthus bioassays). 6(2,5-Dihydroxybenzylamino)purine (LGR-991) was identified as a cytokinin receptor antagonist. At the molecular level LGR-991 blocks the cytokinin receptor CRE1/AHK4 with the same potency as PI-55.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2011

Number of the records: 1