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Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species
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SYSNO ASEP 0347998 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species Author(s) Luo, M. C. (US)
Ma, Y. Q. (US)
You, F. M. (US)
Anderson, O. D. (US)
Kopecký, David (UEB-Q) RID, ORCID
Šimková, Hana (UEB-Q) RID, ORCID
Šafář, Jan (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCID
Gill, B. (US)
McGuire, P. E. (US)
Dvorak, J. (US)Source Title BMC Genomics. - : BioMed Central - ISSN 1471-2164
Roč. 11, č. 122 (2010), s. 1-8Number of pages 8 s. Language eng - English Country US - United States Keywords BREAD WHEAT ; TETRAPLOID WHEAT ; GENETIC-MAP Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000275628900001 DOI 10.1186/1471-2164-11-122 Annotation The presence of closely related genomes in polyploid species makes the assembly of total genomic sequence from shotgun sequence reads produced by the current sequencing platforms exceedingly difficult, if not impossible. Genomes of polyploid species could be sequenced following the ordered clone sequencing approach employing contigs of bacterial artificial chromosome (BAC) clones and BAC-based physical maps. In this work, physical mapping utilizing the SNaPshot HICF of BAC libraries of allohexaploid common wheat in which it is possible to construct single-chromosome or single-chromosome-arm BAC libraries from DNA of flow-sorted chromosomes and bypass the obstacles created by polyploidy. Physical maps of equally good BAC contigs, specific for three wheat single-chromosome libraries (3B, 3AS and 3DS), were constructed in this work. Because of the high purity of the resulting assembled contigs, they can be directly used for genome sequencing. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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