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Production of Polyclonal Antibodies to Potato virus X Using Recombinant Coat Protein

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    SYSNO ASEP0347697
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleProduction of Polyclonal Antibodies to Potato virus X Using Recombinant Coat Protein
    Author(s) Čeřovská, Noemi (UEB-Q) RID, ORCID
    Moravec, Tomáš (UEB-Q) RID, ORCID
    Plchová, Helena (UEB-Q) RID, ORCID
    Hoffmeisterová, Hana (UEB-Q) RID
    Kmoníčková, Jitka (UEB-Q)
    Dědič, P. (CZ)
    Source TitleJournal of Phytopathology - Phytopathologische Zeitschrift - ISSN 0931-1785
    Roč. 158, č. 1 (2010), s. 66-68
    Number of pages3 s.
    Languageeng - English
    CountryDE - Germany
    KeywordsPotato virus X ; recombinant viral antigen ; antibodies
    Subject RIVEB - Genetics ; Molecular Biology
    R&D Projects1M06030 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    CEZAV0Z50380511 - UEB-Q (2005-2011)
    UT WOS000272582000011
    DOI10.1111/j.1439-0434.2009.01580.x
    AnnotationPolyclonal antibodies to recombinant Potato virus X (PVX) coat protein (PVX-CP) were developed and their effectiveness determined in different ELISA protocols. The PVX-CP gene was amplified by reverse transcription-polymerase chain reaction, cloned and expressed in Escherichia coli. For immunization, the CP fractions from bacterial lysate were purified either by simple fractionation or by excision from sodium dodecyl sulphate gels. The PVX-CP was injected into rabbits for antibody production. The PVX-CP antibodies reacted in an indirect plate trapped antigen enzyme-linked immunosorbent assay and immunoblot assay and were useful for the detection of a broad spectrum of isolates of PVX.
    WorkplaceInstitute of Experimental Botany
    ContactDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Year of Publishing2011
Number of the records: 1