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Production of Polyclonal Antibodies to Potato virus X Using Recombinant Coat Protein
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SYSNO ASEP 0347697 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Production of Polyclonal Antibodies to Potato virus X Using Recombinant Coat Protein Author(s) Čeřovská, Noemi (UEB-Q) RID, ORCID
Moravec, Tomáš (UEB-Q) RID, ORCID
Plchová, Helena (UEB-Q) RID, ORCID
Hoffmeisterová, Hana (UEB-Q) RID
Kmoníčková, Jitka (UEB-Q)
Dědič, P. (CZ)Source Title Journal of Phytopathology - Phytopathologische Zeitschrift. - : Wiley - ISSN 0931-1785
Roč. 158, č. 1 (2010), s. 66-68Number of pages 3 s. Language eng - English Country DE - Germany Keywords Potato virus X ; recombinant viral antigen ; antibodies Subject RIV EB - Genetics ; Molecular Biology R&D Projects 1M06030 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000272582000011 DOI 10.1111/j.1439-0434.2009.01580.x Annotation Polyclonal antibodies to recombinant Potato virus X (PVX) coat protein (PVX-CP) were developed and their effectiveness determined in different ELISA protocols. The PVX-CP gene was amplified by reverse transcription-polymerase chain reaction, cloned and expressed in Escherichia coli. For immunization, the CP fractions from bacterial lysate were purified either by simple fractionation or by excision from sodium dodecyl sulphate gels. The PVX-CP was injected into rabbits for antibody production. The PVX-CP antibodies reacted in an indirect plate trapped antigen enzyme-linked immunosorbent assay and immunoblot assay and were useful for the detection of a broad spectrum of isolates of PVX. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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