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Immunogenicity of dendritic cell-based HPV16 E6/E7 peptide vaccines: CTL activation and protective effects
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SYSNO ASEP 0108994 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Immunogenicity of dendritic cell-based HPV16 E6/E7 peptide vaccines: CTL activation and protective effects Title Imunogenicita HPV16 E6/E7 peptidových vakcín odvozených z dendritických buněk: aktivace a obranný účinek CTL Author(s) Indrová, Marie (UMG-J) RID
Reiniš, Milan (UMG-J) RID
Bubeník, Jan (UMG-J)
Jandlová, Táňa (UMG-J)
Bieblová, Jana (UMG-J)
Vonka, V. (CZ)
Velek, Jiří (UOCHB-X)Source Title Folia Biologica. - : Univerzita Karlova v Praze - ISSN 0015-5500
Roč. 50, č. 6 (2004), s. 184-193Number of pages 10 s. Language eng - English Country CZ - Czech Republic Keywords HPV16 E6/E7 peptides ; dendritic cells ; cytotoxic lymphocytes Subject RIV EC - Immunology R&D Projects NC7148 GA MZd - Ministry of Health (MZ) NR7807 GA MZd - Ministry of Health (MZ) GA301/01/0985 GA ČR - Czech Science Foundation (CSF) GA301/04/0492 GA ČR - Czech Science Foundation (CSF) KSK5011112 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z5052915 - UMG-J Annotation We have investigated the capacity of cellular vaccines based on dendritic cells loaded with human HPV16 E6/E7 oncoprotein-derived peptides to induce immune responses in vitro and to elicit protective immunity in a murine experimental model mimicking human HPV16-associated carcinomas. We have found a good correlation between the vaccine efficacy in vitro, determined by chromium release cytotoxic tests, and the level of protection against tumour growth in immunization-challenge experiments. The results from both in vitro and in vivo experiments revealed that BMDC-based cellular vaccines pulsed with peptides, which exceed the length optimal for binding of the MHC class I and which harbour the Th epitope, are capable to elicit strong and protective CTL-based immunity against HPV16-associated MHC class I positive tumours Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2005
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