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Isolation of phages infecting the abundant freshwater iActinobacteriota/i order 'iCa/i. Nanopelagicales'
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SYSNO ASEP 0583806 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Isolation of phages infecting the abundant freshwater iActinobacteriota/i order 'iCa/i. Nanopelagicales' Author(s) Kavagutti, Vinicius Silva (BC-A) RID, ORCID
Chiriac, Maria-Cecilia (BC-A) RID
Ghai, Rohit (BC-A) RID, ORCID
Salcher, Michaela M. (BC-A) RID, ORCID
Haber, Markus (BC-A) RIDNumber of authors 5 Source Title The ISME Journal. - : Nature Publishing Group - ISSN 1751-7362
Roč. 17, č. 6 (2023), s. 943-946Number of pages 4 s. Language eng - English Country GB - United Kingdom Keywords protein ; genomes ; phages Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA21-21990S GA ČR - Czech Science Foundation (CSF) GA23-06806S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000951901900001 EID SCOPUS 85150644444 DOI 10.1038/s41396-023-01400-5 Annotation Low-GC Actinobacteriota of the order 'Ca. Nanopelagicales' (also known as acI or hgcI clade) are abundant in freshwaters around the globe. Extensive predation pressure by phages has been assumed to be the reason for their high levels of microdiversity. So far, however, only a few metagenome-assembled phages have been proposed to infect them and no phages have been isolated. Taking advantage of recent advances in the cultivation of 'Ca. Nanopelagicales' we isolated a novel species of its genus 'Ca. Planktophila'. Using this isolate as bait, we cultivated the first two phages infecting this abundant bacterial order. Both genomes contained a whiB-like transcription factor and a RNA polymerase sigma-70 factor, which might aid in manipulating their host's metabolism. Both phages encoded a glycosyltransferase and one an anti-restriction protein, potential means to evade degradation of their DNA by nucleases present in the host genome. The two phage genomes shared only 6% of their genome with their closest relatives, with whom they form a previously uncultured family of actinophages within the Caudoviricetes. Read recruitment analyses against globally distributed metagenomes revealed the endemic distribution of this group of phages infecting 'Ca. Nanopelagicales'. The recruitment pattern against metagenomes from the isolation site and the modular distribution of shared genes between the two phages indicate high levels of horizontal gene transfer, likely mirroring the microdiversity of their host in the evolutionary arms race between host and phage. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2024 Electronic address https://doi.org/10.1038/s41396-023-01400-5
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