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Microtubules under mechanical pressure can breach dense actin networks
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SYSNO ASEP 0583048 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Microtubules under mechanical pressure can breach dense actin networks Author(s) Gelin, M. (FR)
Schaeffer, A. (FR)
Gaillard, J. (FR)
Guerin, C. (FR)
Vianay, B. (FR)
Orhant-Prioux, M. (FR)
Braun, Marcus (BTO-N) ORCID
Leterrier, C. (FR)
Blanchoin, L. (FR)
Thery, M. (FR)Number of authors 10 Article number jcs261667 Source Title Journal of Cell Science. - : Company of Biologists - ISSN 0021-9533
Roč. 136, č. 22 (2023)Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords dynamic instability ; growth ; migration ; complex ; protein ; populations ; nucleation ; filopodia ; filaments ; Lipid Subject RIV EB - Genetics ; Molecular Biology OECD category Cell biology Method of publishing Open access Institutional support BTO-N - RVO:86652036 UT WOS 001124512500003 EID SCOPUS 85178536110 DOI 10.1242/jcs.261667 Annotation The crosstalk between the actin network and microtubules is essential for cell polarity. It orchestrates microtubule organization within the cell, driven by the asymmetry of actin architecture along the cell periphery. The physical intertwining of these networks regulates spatial organization and force distribution in the microtubule network. Although their biochemical interactions are becoming clearer, the mechanical aspects remain less understood. To explore this mechanical interplay, we developed an in vitro reconstitution assay to investigate how dynamic microtubules interact with various actin filament structures. Our findings revealed that microtubules can align and move along linear actin filament bundles through polymerization force. However, they are unable to pass through when encountering dense branched actin meshworks, similar to those present in the lamellipodium along the periphery of the cell. Interestingly, immobilizing microtubules through crosslinking with actin or other means allow the buildup of pressure, enabling them to breach these dense actin barriers. This mechanism offers insights into microtubule progression towards the cell periphery, with them overcoming obstacles within the denser parts of the actin network and ultimately contributing to cell polarity establishment. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2024 Electronic address https://journals.biologists.com/jcs/article/136/22/jcs261667/335502/Microtubules-under-mechanical-pressure-can-breach
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