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Physiological and transcriptome profiling of Chlorella sorokiniana: A study on azo dye wastewater decolorization
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SYSNO ASEP 0579195 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Physiological and transcriptome profiling of Chlorella sorokiniana: A study on azo dye wastewater decolorization Author(s) Tarbajová, V. (CZ)
Koláčková, M. (CZ)
Chaloupsky, P. (CZ)
Dobešová, M. (CZ)
Cápal, Petr (UEB-Q) RID, ORCID
Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Samek, Ota (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Švec, P. (CZ)
Skopalová Štěrbová, D. (CZ)
Vaculovičová, M. (CZ)
Richtera, L. (CZ)
Pérez-de-Mora, A. (DE)
Adam, V. (CZ)
Húska, D. (CZ)Number of authors 15 Article number 132450 Source Title Journal of Hazardous Materials. - : Elsevier - ISSN 0304-3894
Roč. 460, 15 October (2023)Number of pages 12 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords pigment ; Microalgae ; bioremediation ; RNA-seq ; Raman micro-spectroscopy ; Fourier transform infrared spectroscopy Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Method of publishing Limited access Institutional support UPT-D - RVO:68081731 ; UEB-Q - RVO:61389030 UT WOS 001163085400001 EID SCOPUS 85170692338 DOI 10.1016/j.jhazmat.2023.132450 Annotation Over decades, synthetic dyes have become increasingly dominated by azo dyes posing a significant environmental risk due to their toxicity. Microalgae-based systems may offer an alternative for treatment of azo dye effluents to conventional physical-chemical methods. Here, microalgae were tested to decolorize industrial azo dye wastewater (ADW). Chlorella sorokiniana showed the highest decolorization efficiency in a preliminary screening test. Subsequently, the optimization of the experimental design resulted in 70% decolorization in a photobioreactor. Tolerance of this strain was evidenced using multiple approaches (growth and chlorophyll content assays, scanning electron microscopy (SEM), and antioxidant level measurements). Raman microspectroscopy was employed for the quantification of ADW-specific compounds accumulated by the microalgal biomass. Finally, RNA-seq revealed the transcriptome profile of C. sorokiniana exposed to ADW for 72 h. Activated DNA repair and primary metabolism provided sufficient energy for microalgal growth to overcome the adverse toxic conditions. Furthermore, several transporter genes, oxidoreductases-, and glycosyltransferases-encoding genes were upregulated to effectively sequestrate and detoxify the ADW. This work demonstrates the potential utilization of C. sorokiniana as a tolerant strain for industrial wastewater treatment, emphasizing the regulation of its molecular mechanisms to cope with unfavorable growth conditions. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2024 Electronic address https://www.sciencedirect.com/science/article/pii/S0304389423017338
Number of the records: 1