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Autologous Mesenchymal Stromal Cells Immobilized in Plasma-Based Hydrogel for the Repair of Articular Cartilage Defects in a Large Animal Model
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SYSNO ASEP 0576982 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Autologous Mesenchymal Stromal Cells Immobilized in Plasma-Based Hydrogel for the Repair of Articular Cartilage Defects in a Large Animal Model Author(s) Berounský, K. (CZ)
Vacková, Irena (FGU-C) ORCID, RID
Vištejnová, L. (CZ)
Malečková, A. (CZ)
Havránková, J. (CZ)
Klein, P. (CZ)
Kolinko, Y. (CZ)
Petrenko, Yuriy (FGU-C) ORCID, RID, SAI
Pražák, Šimon (FGU-C) ORCID
Hanák, F. (CZ)
Přidal, J. (CZ)
Havlas, V. (CZ)Number of authors 12 Source Title Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 72, č. 4 (2023), s. 485-495Number of pages 11 s. Language eng - English Country CZ - Czech Republic Keywords bone marrow mesenchymal stromal cells ; scaffold osteochondral defect ; minipig ; implantation OECD category Biomaterials (as related to medical implants, devices, sensors) R&D Projects NV19-06-00355 GA MZd - Ministry of Health (MZ) Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 001078316100007 EID SCOPUS 85173004424 DOI 10.33549/physiolres.935098 Annotation The treatment of cartilage defects in trauma injuries and degenerative diseases represents a challenge for orthopedists. Advanced mesenchymal stromal cell (MSC)-based therapies are currently of interest for the repair of damaged cartilage. However, an approved system for MSC delivery and maintenance in the defect is still missing. This study aimed to evaluate the effect of autologous porcine bone marrow MSCs anchored in a commercially available polyglycolic acid-hyaluronan scaffold (Chondrotissue (R)) using autologous blood plasma-based hydrogel in the repair of osteochondral defects in a large animal model. The osteochondral defects were induced in twenty-four minipigs with terminated skeletal growth. Eight animals were left untreated, eight were treated with Chondrotissue (R) and eight received Chondrotissue (R) loaded with MSCs. The animals were terminated 90 days after surgery. Macroscopically, the untreated defects were filled with newly formed tissue to a greater extent than in the other groups. The histological evaluations showed that the defects treated with Chondrotissue (R) and Chondrotissue (R) loaded with pBMSCs contained a higher amount of hyaline cartilage and a lower amount of connective tissue, while untreated defects contained a higher amount of connective tissue and a lower amount of hyaline cartilage. In addition, undifferentiated connective tissue was observed at the edges of defects receiving Chondrotissue (R) loaded with MSCs, which may indicate the extracellular matrix production by transplanted MSCs. The immunological analysis of the blood samples revealed no immune response activation by MSCs application. This study demonstrated the successful and safe immobilization of MSCs in commercially available scaffolds and defect sites for cartilage defect repair. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2024 Electronic address https://www.biomed.cas.cz/physiolres/pdf/2023/72_485.pdf
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