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The Influence of Calcium on the Growth, Morphology and Gene Regulation in Gemmatimonas phototrophica
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SYSNO ASEP 0571792 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Influence of Calcium on the Growth, Morphology and Gene Regulation in Gemmatimonas phototrophica Author(s) Shivaramu, Sahana (MBU-M)
Tomasch, Jurgen (MBU-M) ORCID, RID
Kopejtka, Karel (MBU-M) ORCID, RID
Nupur, Nupur (MBU-M) ORCID
Saini, Mohit Kumar (MBU-M) ORCID, RID
Bokhari, Syed Nadeem Hussain (BC-A) RID
Küpper, Hendrik (BC-A) RID, ORCID
Koblížek, Michal (MBU-M) RID, ORCIDArticle number 27 Source Title Microorganisms. - : MDPI
Roč. 11, č. 1 (2023)Number of pages 15 s. Language eng - English Country CH - Switzerland Keywords Gemmatimonas phototrophica ; anoxygenic phototrophic bacteria ; horizontal gene transfer ; calcium ; transcriptomics Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GX19-28778X GA ČR - Czech Science Foundation (CSF) EF15_003/0000336 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 ; BC-A - RVO:60077344 UT WOS 000918288200001 EID SCOPUS 85146759617 DOI 10.3390/microorganisms11010027 Annotation The bacterium Gemmatimonas phototrophica AP64 isolated from a freshwater lake in the western Gobi Desert represents the first phototrophic member of the bacterial phylum Gemmatimonadota. This strain was originally cultured on agar plates because it did not grow in liquid medium. In contrast, the closely related species G. groenlandica TET16 grows both on solid and in liquid media. Here, we show that the growth of G. phototrophica in liquid medium can be induced by supplementing the medium with 20 mg CaCl2 L-1. When grown at a lower concentration of calcium (2 mg CaCl2 L-1) in the liquid medium, the growth was significantly delayed, cells were elongated and lacked flagella. The elevated requirement for calcium is relatively specific as it can be partially substituted by strontium, but not by magnesium. The transcriptome analysis documented that several groups of genes involved in flagella biosynthesis and transport of transition metals were co-activated after amendment of 20 mg CaCl2 L-1 to the medium. The presented results document that G. phototrophica requires a higher concentration of calcium for its metabolism and growth compared to other Gemmatimonas species. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2024 Electronic address https://www.mdpi.com/2076-2607/11/1/27
Number of the records: 1