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Fate of the capping agent of biologically produced gold nanoparticles and adsorption of enzymes onto their surface
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SYSNO ASEP 0571151 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Fate of the capping agent of biologically produced gold nanoparticles and adsorption of enzymes onto their surface Author(s) Pourali, Parastoo (MBU-M)
Dzmitruk, Volha (BTO-N)
Pátek, Miroslav (MBU-M) RID, ORCID
Neuhöferová, Eva (MBU-M)
Svoboda, Milan (UIACH-O) RID, ORCID
Benson, Veronika (MBU-M) RID, ORCIDArticle number 4916 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 13, č. 1 (2023)Number of pages 12 s. Language eng - English Country US - United States Keywords Adsorption ; Endopeptidase K ; Gold ; Metal Nanoparticles ; Ribonuclease ; Pancreatic ; gold ; metal nanoparticle ; pancreatic ribonuclease ; proteinase K ; adsorption ; chemistry OECD category Microbiology Subject RIV - cooperation Institute of Analytical Chemistry - Analytical Chemistry, Separation R&D Projects EH22_010/0002357 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2023042 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF18_046/0015974 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i. Method of publishing Open access Institutional support MBU-M - RVO:61388971 ; BTO-N - RVO:86652036 ; UIACH-O - RVO:68081715 UT WOS 001017313600007 EID SCOPUS 85150969884 DOI 10.1038/s41598-023-31792-5 Annotation Enzymotherapy based on DNase I or RNase A has often been suggested as an optional strategy for cancer treatment. The efficacy of such procedures is limited e.g. by a short half-time of the enzymes or a low rate of their internalization. The use of nanoparticles, such as gold nanoparticles (AuNPs), helps to overcome these limits. Specifically, biologically produced AuNPs represent an interesting variant here due to naturally occurring capping agents (CA) on their surface. The composition of the CA depends on the producing microorganism. CAs are responsible for the stabilization of the nanoparticles, and promote the direct linking of targeting and therapeutic molecules. This study provided proof of enzyme adsorption onto gold nanoparticles and digestion efficacy of AuNPs-adsorbed enzymes. We employed Fusarium oxysporum extract to produce AuNPs. These nanoparticles were round or polygonal with a size of about 5 nm, negative surface charge of about − 33 mV, and maximum absorption peak at 530 nm. After the adsorption of DNAse I, RNase A, or Proteinase K onto the AuNPs surface, the nanoparticles exhibited shifts in surface charge (values between − 22 and − 13 mV) and maximum absorption peak (values between 513 and 534 nm). The ability of AuNP-enzyme complexes to digest different targets was compared to enzymes alone. We found a remarkable degradation of ssDNA, and dsDNA by AuNP-DNAse I, and a modest degradation of ssRNA by AuNP-RNase A. The presence of particular enzymes on the AuNP surface was proved by liquid chromatography–mass spectrometry (LC–MS). Using SDS-PAGE electrophoresis, we detected a remarkable digestion of collagen type I and fibrinogen by AuNP-proteinase K complexes. We concluded that the biologically produced AuNPs directly bound DNase I, RNase A, and proteinase K while preserving their ability to digest specific targets. Therefore, according to our results, AuNPs can be used as effective enzyme carriers and the AuNP-enzyme conjugates can be effective tools for enzymotherapy. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2024 Electronic address https://www.nature.com/articles/s41598-023-31792-5
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