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Polyelectrolyte nanoparticles based on poly[N-(2-hydroxypropyl)methacrylamide-block-poly(N-(3-aminopropyl)methacrylamide] copolymers for delivery of heparin-binding proteins
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SYSNO ASEP 0570910 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Polyelectrolyte nanoparticles based on poly[N-(2-hydroxypropyl)methacrylamide-block-poly(N-(3-aminopropyl)methacrylamide] copolymers for delivery of heparin-binding proteins Author(s) García-Briones, Gabriela S. (UMCH-V)
Laga, Richard (UMCH-V) RID, ORCID
Černochová, Zulfiya (UMCH-V) RID, ORCID
Arjona-Ruiz, Carely (UMCH-V)
Janoušková, Olga (UMCH-V) RID, SAI, ORCID
Šlouf, Miroslav (UMCH-V) RID, ORCID
Pop-Georgievski, Ognen (UMCH-V) RID, ORCID
Kubies, Dana (UMCH-V) RID, ORCIDArticle number 111976 Source Title European Polymer Journal. - : Elsevier - ISSN 0014-3057
Roč. 191, 13 June (2023)Number of pages 15 s. Language eng - English Country GB - United Kingdom Keywords polyelectrolyte nanoparticles ; APMA ; heparin Subject RIV CD - Macromolecular Chemistry OECD category Polymer science R&D Projects GA23-06746S GA ČR - Czech Science Foundation (CSF) GA22-02836S GA ČR - Czech Science Foundation (CSF) LTAUSA18173 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LX22NPO5102 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UMCH-V - RVO:61389013 UT WOS 000979460600001 EID SCOPUS 85151482989 DOI 10.1016/j.eurpolymj.2023.111976 Annotation In addition to the delivery of therapeutic nucleic acids and low-molecular weight drugs, polyelectrolyte nanoparticles (NPs) have recently been studied as carriers for protein delivery. However, the stability of NPs during isolation steps, which ensures their easy redispersion, needs to be solved empirically for individual systems using cryoprotectants and stabilizers. To avoid the use of additives, we studied the formation of polyelectrolyte NPs consisting of a newly synthesized polycationic diblock copolymer based on poly(N-(2-hydroxypropyl)methacrylamide)-block-poly(N-(3-aminopropyl)methacrylamide) (p(HPMA-b-APMA) and heparin (Hep). The p(APMA) blocks electrostatically complexed with Hep, and the p(HPMA) blocks formed a neutral corona of NPs, limiting NP aggregation. Self-assembly was monitored through the changes in size and zeta potential of the formed NPs, which depended on the copolymer composition and the concentration of polyelectrolyte solutions. The interactions between the NP components were analysed by FTIR spectroscopy, and XPS analysis indicated the presence of p(HPMA) blocks on the surface of the NPs. The encapsulation of the model chemokine CXCL12 and basic fibroblast growth factor (FGF-2) was driven by their specific bioaffinity for Hep, resulting in a high 90 % entrapment efficiency and increased protein stability. CXCL12 was released over 48 h, while FGF-2 exhibited a sustained release of up to 38 % over four weeks. In addition, the released CXCL12 effectively stimulated the migration of macrophages and T-lymphocyte cells, indicating the preserved protein bioactivity. Considering the proven noncytotoxic performance of the NPs towards fibroblasts and mesenchymal stem cells, the polyelectrolyte NPs of p(HPMA-b-APMA) and Hep loaded with heparin-binding proteins can be considered as promising candidates for the controlled delivery of bioactive proteins in biomedical applications. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2024 Electronic address https://www.sciencedirect.com/science/article/pii/S0014305723001593?via%3Dihub
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