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Microstructural Analysis of Collagenous Structures in Relapsed Clubfoot Tissue
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SYSNO ASEP 0570504 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Microstructural Analysis of Collagenous Structures in Relapsed Clubfoot Tissue Author(s) Vondrášek, David (FGU-C) ORCID, SAI
Hadraba, Daniel (FGU-C) RID, ORCID, SAI
Přibyl, J. (CZ)
Eckhardt, Adam (FGU-C) RID, ORCID
Ošťádal, M. (CZ)
Lopot, F. (CZ)
Jelen, K. (CZ)
Doubková, Martina (FGU-C) ORCID, RID
Knitlová, Jarmila (FGU-C) RID, ORCID
Novotný, T. (CZ)
Janáček, Jiří (FGU-C) RID, ORCIDSource Title Microscopy and Microanalysis. - : Cambridge University Press - ISSN 1431-9276
Roč. 29, č. 1 (2023), s. 265-272Number of pages 8 s. Language eng - English Country US - United States Keywords atomic force microscopy ; label-free imaging ; mechanical properties ; multi-modal analysis ; orthopedic surgery ; relapsed clubfoot ; talipes equinovarus congenitus OECD category Orthopaedics R&D Projects NV17-31564A GA MZd - Ministry of Health (MZ) NU22-10-00072 GA MZd - Ministry of Health (MZ) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF18_046/0016045 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure CIISB II - 90127 - Masarykova univerzita Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 001033590800026 EID SCOPUS 85153729727 DOI 10.1093/micmic/ozac012 Annotation Talipes equinovarus congenitus (clubfoot) is frequently defined as a stiff, contracted deformity, but few studies have described the tissue from the point of view of the extracellular matrix, and none have quantified its mechanical properties. Several researchers have observed that clubfoot exhibits signs of fibrosis in the medial side of the deformity that are absent in the lateral side. Our study aims to quantify the differences between the medial and lateral side tissue obtained from relapsed clubfoot during surgery in terms of the morphological and mechanical properties of the tissue. Combining methods of optical and atomic force microscopy, our study revealed that the medial side has a higher Young’s modulus, contains more collagen and less adipose tissue and that the collagen fibers propagate at a higher frequency of the crimp pattern after surgical dissection of the tissue. Our study offers a multi-correlative approach that thoroughly investigates the relapsed clubfoot tissue. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2024 Electronic address https://doi.org/10.1093/micmic/ozac012
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