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Characterization of insulin crystalline form in isolated β-cell secretory granules
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SYSNO ASEP 0566032 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Characterization of insulin crystalline form in isolated β-cell secretory granules Author(s) Asai, Seiya (UOCHB-X) ORCID
Moravcová, J. (CZ)
Žáková, Lenka (UOCHB-X) RID, ORCID
Selicharová, Irena (UOCHB-X) RID, ORCID
Hadravová, Romana (UOCHB-X) RID, ORCID
Brzozowski, A. M. (GB)
Nováček, J. (CZ)
Jiráček, Jiří (UOCHB-X) RID, ORCIDArticle number 220322 Source Title Open Biology. - : Royal Society Publishing
Roč. 12, č. 12 (2022)Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords insulin secretory granules ; protein crystals ; in vivo crystalization ; cryo-EM OECD category Cell biology R&D Projects EF16_019/0000729 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure CIISB II - 90127 - Masarykova univerzita Method of publishing Open access Institutional support UOCHB-X - RVO:61388963 UT WOS 000900974200003 EID SCOPUS 85144273379 DOI 10.1098/rsob.220322 Annotation Insulin is stored in vivo inside the pancreatic β-cell insulin secretory granules. However, the structural features of the storage forms of insulin in native preparations of secretory granules are unknown, because of their small size, fragile character and difficult handling. We isolated and investigated the secretory granules from MIN6 cells under near-native conditions, using cryo-electron microscopic (Cryo-EM) techniques. Our experimental data show that insulin can be pre-sent in pancreatic MIN6 cell granules in a microcrystalline form, probably consisting of 4Zn2+-hexamers of this hormone Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2023 Electronic address https://doi.org/10.1098/rsob.220322
Number of the records: 1