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Bio-production of eicosapentaenoic acid from the diatom Nanofrustulum shiloi via two-step high performance countercurrent chromatography
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SYSNO ASEP 0565820 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Bio-production of eicosapentaenoic acid from the diatom Nanofrustulum shiloi via two-step high performance countercurrent chromatography Author(s) Bárcenas-Pérez, Daniela (MBU-M) ORCID, RID
Lukeš, Martin (MBU-M) ORCID
Hrouzek, Pavel (MBU-M) ORCID
Zápal, Jakub (MBU-M)
Kuzma, Marek (MBU-M) ORCID, RID
Kopecký, Jiří (MBU-M) ORCID
Kubáč, David (MBU-M) RID, ORCID
Arredondo-Vega, B. O. (MX)
Cheel Horna, José (MBU-M)Source Title Journal of Applied Phycology. - : Springer - ISSN 0921-8971
Roč. 34, SEP 1 (2022), s. 2995-3010Number of pages 16 s. Language eng - English Country US - United States Keywords Eicosapentaenoic acid ; Nanofrustulum shiloi ; Diatom ; High performance countercurrent chromatography (HPCCC) Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects TN01000048 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000847965100001 EID SCOPUS 85137249326 DOI 10.1007/s10811-022-02816-w Annotation Eicosapentaenoic acid (EPA) is an essential polyunsaturated fatty acid of nutritional and physiological relevance for humans. It is commercially produced mainly from fish oil, but new sustainable sources are required. In this study, pure EPA ethyl ester was produced from Nanofrustulum shiloi biomass by integrating two separation steps via high performance countercurrent chromatography (HPCCC). The HPCCC first step consisted of a 10 separation cycles (1200 mg sample per cycle) process, each cycle consisting of three stages comprising EPA fraction separation in reversed phase elution mode (lower polar phase used as mobile phase), with stationary phase extrusion (by switching the pumping of the mobile phase to the stationary phase, while maintaining the column rotation), and hydrodynamic equilibrium achievement inside the column. The process led to 982.1 mg of a fraction rich in EPA with a purity of 71%. To improve the purity of the EPA fraction, an HPCCC second step was performed leading to 698.97 mg of EPA ethyl ester with a purity of 99%, and a recovery of 98%. To avoid unnecessary waste of solvents, liquid phases were formulated based on a priori NMR measurement. The HPCCC process throughput was 0.936 g h(-1), while the efficiency per gram of EPA was 0.054 g h(-1). Environmental risk and process evaluation factors were used for the evaluation of the separation process. Overall, this isolation approach may represent a useful model for the efficient production of pure EPA from microalgae. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://link.springer.com/article/10.1007/s10811-022-02816-w
Number of the records: 1