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Surface modifications of a silicalite film designed for coating orthopaedic implants

SYSNO ASEP	0565677
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Surface modifications of a silicalite film designed for coating orthopaedic implants
Author(s)	Brož, Antonín (FGU-C)_{RID, ORCID, SAI} Jirka, Ivan (UFCH-W)_{RID, ORCID} Matějka, Roman (FGU-C)_{RID, ORCID, SAI} Štěpanovská, Jana (FGU-C)_{ORCID, RID} Doubková, Martina (FGU-C)_{ORCID, RID} Sajdl, P. (CZ) Drahokoupil, Jan (FZU-D)_{RID, ORCID} Volochanskyi, Oleksandr (UFCH-W)_{ORCID, SAI} Futóová, Terézia (FGU-C) Bačáková, Lucie (FGU-C)_{RID, ORCID}
Article number	111373
Source Title	Materials and Design. - : Elsevier - ISSN 0264-1275 Roč. 224, Dec (2022)
Number of pages	13 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	silicalite ; synthesis ; biocompatibility ; surface modification ; cell differentiation ; implants
OECD category	Biomaterials (as related to medical implants, devices, sensors)
R&D Projects	GA19-02891S GA ČR - Czech Science Foundation (CSF)
	LM2018124 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823 ; UFCH-W - RVO:61388955 ; FZU-D - RVO:68378271
UT WOS	000891761700003
EID SCOPUS	85142184888
DOI	10.1016/j.matdes.2022.111373
Annotation	The effect of various surface modifications of silicalite-1 film grown on a Ti6Al4V alloy on biocompatibility and cell differentiation has been addressed. The as-synthesized silicalite film was first modified by alkaline etching and by subsequent treatments in oxygen radiofrequency (O2 RF) plasma, by calcination at 500 °C, and by processing the calcinated samples in the O2 RF plasma. The chemical composition, crystallinity, topography and wettability of samples were characterized using angle-resolved X-ray photoelectron spectroscopy, X-ray diffraction, scanning electron microscopy, and sessile water drop contact angle measurements. We have discovered the presence of a non-zeolitic defective SiOx phase (NZP) with coordinated hydrocarbon chains. The NZP can be removed by the exposition to the O2 RF plasma. The metabolic activity tests done on human osteoblast line Saos-2 revealed that the NZP can influence cell proliferation, however, all studied modifications of silicalite film can be considered biocompatible. The osteogenic differentiation was studied on human mesenchymal stem cells under static conditions and under dynamic (pulsatile and shear) stress. The results obtained by the real-time qPCR and immunofluorescence microscopy suggest that etched, plasma-treated and calcinated + plasma-treated samples are the most promising for osteogenic cell differentiation.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2023
Electronic address	https://doi.org/10.1016/j.matdes.2022.111373

Number of the records: 1