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Raman Spectroscopy - A Novel Method for Identification and Characterization of Microbes on a Single-Cell Level in Clinical Settings
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SYSNO ASEP 0565445 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Raman Spectroscopy - A Novel Method for Identification and Characterization of Microbes on a Single-Cell Level in Clinical Settings Author(s) Rebrošová, K. (CZ)
Samek, Ota (UPT-D) RID, ORCID, SAI
Kizovský, Martin (UPT-D)
Bernatová, Silvie (UPT-D) RID, SAI
Holá, V. (CZ)
Růžička, F. (CZ)Number of authors 6 Article number 866463 Source Title Frontiers in Cellular and Infection Microbiology. - : Frontiers Media - ISSN 2235-2988
Roč. 12, 22 April (2022)Number of pages 10 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords Raman spectroscopy ; Raman tweezers ; identification of microorganisms ; antimicrobial resistance ; microfluidic devices ; magnetic beads ; diagnostics Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) R&D Projects NU21-05-00341 GA MZd - Ministry of Health (MZ) Method of publishing Open access Institutional support UPT-D - RVO:68081731 UT WOS 000810475900001 EID SCOPUS 85129652774 DOI 10.3389/fcimb.2022.866463 Annotation Rapid and accurate identification of pathogens causing infections is one of the biggest challenges in medicine. Timely identification of causative agents and their antimicrobial resistance profile can significantly improve the management of infection, lower costs for healthcare, mitigate ever-growing antimicrobial resistance and in many cases, save lives. Raman spectroscopy was shown to be a useful-quick, non-invasive, and non-destructivetool for identifying microbes from solid and liquid media. Modifications of Raman spectroscopy and/or pretreatment of samples allow single-cell analyses and identification of microbes from various samples. It was shown that those non-culture-based approaches could also detect antimicrobial resistance. Moreover, recent studies suggest that a combination of Raman spectroscopy with optical tweezers has the potential to identify microbes directly from human body fluids. This review aims to summarize recent advances in non-culture-based approaches of identification of microbes and their virulence factors, including antimicrobial resistance, using methods based on Raman spectroscopy in the context of possible use in the future point-of-care diagnostic process. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2023 Electronic address https://www.frontiersin.org/articles/10.3389/fcimb.2022.866463/full
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