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Glucose-6-P/phosphate translocator2 mediates the phosphoglucose-isomerase1-independent response to microbial volatiles
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SYSNO ASEP 0565047 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Glucose-6-P/phosphate translocator2 mediates the phosphoglucose-isomerase1-independent response to microbial volatiles Author(s) Gámez-Arcas, S. (ES)
Munoz, F.J. (ES)
Ricarte-Bermejo, A. (ES)
Sánchez-López, Á. M. (ES)
Baslam, M. (ES)
Baroja-Fernández, E. (ES)
Bahaji, A. (ES)
Almagro, G. (ES)
De Diego, N. (CZ)
Doležal, Karel (UEB-Q) RID, ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Leal-López, J. (ES)
Morcillo, R. J. L. (ES)
Castillo, A. G. (GT)
Pozueta-Romero, J. (ES)Number of authors 15 Source Title Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 190, č. 4 (2022), s. 2137-2154Number of pages 18 s. Language eng - English Country US - United States Keywords GLUCOSE 6-PHOSPHATE/PHOSPHATE TRANSLOCATOR ; AUTOCRINE MOTILITY FACTOR ; PHOSPHOGLUCOSE ISOMERASE OECD category Biochemistry and molecular biology R&D Projects EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000861435100001 EID SCOPUS 85142941228 DOI 10.1093/plphys/kiac433 Annotation In Arabidopsis (Arabidopsis thaliana), the plastidial isoform of phosphoglucose isomerase (PGI1) mediates photosynthesis, metabolism, and development, probably due to its involvement in the synthesis of isoprenoid-derived signals in vascular tissues. Microbial volatile compounds (VCs) with molecular masses of <45 Da promote photosynthesis, growth, and starch overaccumulation in leaves through PGI1-independent mechanisms. Exposure to these compounds in leaves enhances the levels of GLUCOSE-6-PHOSPHATE/PHOSPHATE TRANSLOCATOR2 (GPT2) transcripts. We hypothesized that the PGI1-independent response to microbial volatile emissions involves GPT2 action. To test this hypothesis, we characterized the responses of wild-type (WT), GPT2-null gpt2-1, PGI1-null pgi1-2, and pgi1-2gpt2-1 plants to small fungal VCs. In addition, we characterized the responses of pgi1-2gpt2-1 plants expressing GPT2 under the control of a vascular tissue- and root tip-specific promoter to small fungal VCs. Fungal VCs promoted increases in growth, starch content, and photosynthesis in WT and gpt2-1 plants. These changes were substantially weaker in VC-exposed pgi1-2gpt2-1 plants but reverted to WT levels with vascular and root tip-specific GPT2 expression. Proteomic analyses did not detect enhanced levels of GPT2 protein in VC-exposed leaves and showed that knocking out GPT2 reduced the expression of photosynthesis-related proteins in pgi1-2 plants. Histochemical analyses of GUS activity in plants expressing GPT2-GUS under the control of the GPT2 promoter showed that GPT2 is mainly expressed in root tips and vascular tissues around hydathodes. Overall, the data indicated that the PGI1-independent response to microbial VCs involves resetting of the photosynthesis-related proteome in leaves through long-distance GPT2 action. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2023 Electronic address https://doi.org/10.1093/plphys/kiac433
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