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RALF1 peptide triggers biphasic root growth inhibition upstream of auxin biosynthesis
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SYSNO ASEP 0564399 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title RALF1 peptide triggers biphasic root growth inhibition upstream of auxin biosynthesis Author(s) Li, L. (AT)
Chen, H. (GB)
Alotaibi, S. S. (SA)
Pěnčík, Aleš (UEB-Q) ORCID, RID, SAI
Adamowski, M. (AT)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Friml, J. (AT)Number of authors 7 Article number e2121058119 Source Title Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424
Roč. 119, č. 31 (2022)Number of pages 10 s. Language eng - English Country US - United States Keywords auxin ; biphasic regulation ; cross-talk ; ralf1 ; root growth inhibition OECD category Biochemistry and molecular biology R&D Projects EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000881496900002 EID SCOPUS 85135292745 DOI 10.1073/pnas.2121058119 Annotation Plant cell growth responds rapidly to various stimuli, adapting architecture to environmental changes. Two major endogenous signals regulating growth are the phytohormone auxin and the secreted peptides rapid alkalinization factors (RALFs). Both trigger very rapid cellular responses and also exert long-term effects [Du et al., Annu. Rev. Plant Biol. 71, 379–402 (2020), Blackburn et al., Plant Physiol. 182, 1657–1666 (2020)]. However, the way, in which these distinct signaling pathways converge to regulate growth, remains unknown. Here, using vertical confocal microscopy combined with a microfluidic chip, we addressed the mechanism of RALF action on growth. We observed correlation between RALF1-induced rapid Arabidopsis thaliana root growth inhibition and apoplast alkalinization during the initial phase of the response, and revealed that RALF1 reversibly inhibits primary root growth through apoplast alkalinization faster than within 1 min. This rapid apoplast alkalinization was the result of RALF1-induced net H+ influx and was mediated by the receptor FERONIA (FER). Furthermore, we investigated the cross-talk between RALF1 and the auxin signaling pathways during root growth regulation. The results showed that RALF-FER signaling triggered auxin signaling with a delay of approximately 1 h by up-regulating auxin biosynthesis, thus contributing to sustained RALF1-induced growth inhibition. This biphasic RALF1 action on growth allows plants to respond rapidly to environmental stimuli and also reprogram growth and development in the long term. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2023 Electronic address https://doi.org/10.1073/pnas.2121058119
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