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Tuning of surface charge of functionalized poly(carboxybetaine) brushes can significantly improve label-free biosensing in complex media
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SYSNO ASEP 0564268 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Tuning of surface charge of functionalized poly(carboxybetaine) brushes can significantly improve label-free biosensing in complex media Author(s) Víšová, Ivana (FZU-D) ORCID
Houska, Milan (FZU-D) ORCID
Spasovová, Monika (FZU-D) ORCID
Forinová, Michala (FZU-D) ORCID
Pilipenco, Alina (FZU-D) ORCID, RID
Mezuláníková, K. (GB)
Tomandlová, Markéta (FZU-D)
Mrkvová, Kateřina (FZU-D)
Vrabcová, Markéta (FZU-D) ORCID
Dejneka, Alexandr (FZU-D) RID, ORCID
Dostálek, Jakub (FZU-D) ORCID
Vaisocherová-Lísalová, Hana (FZU-D) ORCIDNumber of authors 12 Article number 2201210 Source Title Advanced Materials Interfaces. - : Wiley - ISSN 2196-7350
Roč. 9, č. 33 (2022)Number of pages 7 s. Language eng - English Country DE - Germany Keywords antifouling zwitterionic polymer brushes ; biosensors ; EDC/NHS chemistry ; N-hydroxysuccinimide (NHS) active ester deactivation ; poly(carboxybetaine) functionalization Subject RIV BO - Biophysics OECD category Biophysics R&D Projects GA21-19779S GA ČR - Czech Science Foundation (CSF) EF18_053/0016627 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support FZU-D - RVO:68378271 UT WOS 000845083800001 EID SCOPUS 85136546741 DOI 10.1002/admi.202201210 Annotation Poly(carboxybetaine) brushes are excellent antifouling platforms allowing facile functionalization with biorecognition elements via carbodiimide/N-hydroxysuccin- imide (EDC/NHS) chemistry. However, residual active NHS esters and the loss of zwitterionic balance after the conjugation may impair initially excellent antifouling properties. This problem has so far been addressed either by using spontaneous hydrolysis or deactivation of residual NHS esters by the reaction with a small amino compound bearing hydroxyl or carboxyl groups. In contrast to this approach, and instead of using a single deactivator, here the use of tailored mixtures of deacti- vating agents containing carboxyl groups and sulfo or sulfate groups with perma- nent negative charge that allow to tune surface charge balance is investigated.
Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2023 Electronic address https://doi.org/10.1002/admi.202201210
Number of the records: 1