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Cultivation of the microalgae Chlamydomonas reinhardtii and Desmodesmus quadricauda in highly deuterated media: Balancing the light intensity
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SYSNO ASEP 0563736 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cultivation of the microalgae Chlamydomonas reinhardtii and Desmodesmus quadricauda in highly deuterated media: Balancing the light intensity Author(s) Kselíková, Veronika (MBU-M)
Husarčíková, K. (CZ)
Mojžes, P. (CZ)
Zachleder, Vilém (MBU-M) ORCID
Bišová, Kateřina (MBU-M) RIDNumber of authors 5 Article number 1620816 Source Title Frontiers in Bioengineering and Biotechnology. - : Frontiers Media - ISSN 2296-4185
Roč. 10, SEP 5 2022 (2022)Number of pages 20 s. Language eng - English Country CH - Switzerland Keywords microalgae ; deuterium ; light intensity ; cell division ; deuterated compounds ; physicalstress Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA19-12607S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000874873400001 EID SCOPUS 85138308171 DOI 10.3389/fbioe.2022.960862 Annotation The production of organic deuterated compounds in microalgal systems represents a cheaper and more versatile alternative to more complicated chemical synthesis. In the present study, we investigate the autotrophic growth of two microalgae, Chlamydomonas reinhardtii and Desmodesmus quadricauda, in medium containing high doses of deuterated water, D2O. The growth of such cultures was evaluated in the context of the intensity of incident light, since light is a critical factor in the management of autotrophic algal cultures. Deuteration increases the light sensitivity of both model organisms, resulting in increased levels of singlet oxygen and poorer photosynthetic performance. Our results also show a slowdown in growth and cell division processes with increasing D2O concentrations. At the same time, impaired cell division leads to cell enlargement and accumulation of highly deuterated compounds, especially energy-storing molecules. Thus, considering the specifics of highly deuterated cultures and using the growth conditions proposed in this study, it is possible to obtain highly deuterated algal biomass, which could be a valuable source of deuterated organic compounds. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://www.frontiersin.org/articles/10.3389/fbioe.2022.960862/full
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