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Outdoor photoacclimation of two Chlorella strains characterized by normal and reduced light-harvesting antennas: photosynthetic activity and chlorophyll-protein organization
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SYSNO ASEP 0561863 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Outdoor photoacclimation of two Chlorella strains characterized by normal and reduced light-harvesting antennas: photosynthetic activity and chlorophyll-protein organization Author(s) Masojídek, Jiří (MBU-M) RID, ORCID
Ranglová, Karolína (MBU-M) ORCID
Bečková, Martina (MBU-M) RID
Torzillo, G. (CR)
Knoppová, Jana (MBU-M) RID
Silva Benavides, A. M. (CR)
Charvát, Filip (MBU-M)
Komenda, Josef (MBU-M) RID, ORCIDSource Title Journal of Applied Phycology. - : Springer - ISSN 0921-8971
Roč. 34, č. 5 (2022), s. 2339-2353Number of pages 15 s. Language eng - English Country US - United States Keywords Chlorophyll-protein ; Chlorophyll fluorescence ; Light-harvesting antenna size ; Microalgae ; Oxygen-production ; Photosynthesis Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects TN01000048 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000829689600001 EID SCOPUS 85134763624 DOI 10.1007/s10811-022-02803-1 Annotation Photoacclimation of two Chlorella cultures strain g-120 characterised by a reduced size of light-harvesting antenna complex (LHC) and strain R-117 with full antenna size was studied during 5-day outdoor trials. The aim was to correlate the functional and structural changes in the photosynthetic apparatus to culture growth, photochemical activity and thylakoid composition of chlorophyll (Chl)-protein complexes and corresponding polypeptides. Chlorella g-120 was characterized by a low Chl/biomass ratio (< 0.5% of dry weight), about four times lower compared to Chlorella R-117. The important observation was that the high molecular mass Chl-binding protein supercomplexes, i.e. Photosystem II (PSII) and Photosystem I (PSI) cores associated with LHCs were physically missing or negligible in Chlorella g-120. However, there were no visible changes in Chl-protein composition in the g-120 strain during its acclimation to phototrophic conditions. Measurement of the effective absorption cross-section of PSII centres confirmed a markedly reduced functional antenna size in Chlorella g-120 as compared to R-117 which coincided with the absence of the PSII-LHC supercomplexes. We demonstrated that Chlorella g-120 represents a typical reduced antenna-size strain due to its Chl-protein composition. As compared to the full-antenna Chlorella R-117 strain, the outdoor cultures of Chlorella g-120 showed significantly lower oxygen production and electron transport rate measured in-situ. On the contrary, Chlorella g-120 revealed increased futile energy dissipation via non-photochemical quenching and higher respiration compared to Chlorella R-117. Consequently, the potential use of microalgae strains with reduced LHCII for outdoor mass cultivation may not be as straightforward as anticipated from laboratory experiments. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://link.springer.com/article/10.1007/s10811-022-02803-1
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