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A Plastid-Bound Ankyrin Repeat Protein Controls Gametophyte and Early Embryo Development in Arabidopsis thaliana
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SYSNO ASEP 0561270 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A Plastid-Bound Ankyrin Repeat Protein Controls Gametophyte and Early Embryo Development in Arabidopsis thaliana Author(s) Kulichová, Katarína (UEB-Q) ORCID
Pieters, Janto (UEB-Q) ORCID
Kumar, Vinod (UEB-Q) ORCID
Honys, David (UEB-Q) RID, ORCID
Hafidh, Said (UEB-Q) RID, ORCIDNumber of authors 5 Article number 767339 Source Title Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 13, MAR 8 (2022)Number of pages 16 s. Language eng - English Country CH - Switzerland Keywords embryo development ; fertilization ; pollen ; pollen tube reception ; proplastid OECD category Plant sciences, botany R&D Projects GA21-15856S GA ČR - Czech Science Foundation (CSF) GA22-29717S GA ČR - Czech Science Foundation (CSF) LTAIN19030 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC20028 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000775349600001 EID SCOPUS 85127295001 DOI 10.3389/fpls.2022.767339 Annotation Proplastids are essential precursors for multi-fate plastid biogenesis, including chloroplast differentiation, a powerhouse for photosynthesis in plants. Arabidopsis ankyrin repeat protein (AKRP, AT5G66055) is a plastid-localized protein with a putative function in plastid differentiation and morphogenesis. Loss of function of akrp leads to embryo developmental arrest. Whether AKRP is critical pre-fertilization has remained unresolved. Here, using reverse genetics, we report a new allele, akrp-3, that exhibited a reduced frequency of mutant embryos (<13%) compared to previously reported alleles. akrp-3 affected both male and female gametophytes resulting in reduced viability, incompetence in pollen tube attraction, altered gametic cell fate, and embryo arrest that were depleted of chlorophyll. AKRP is widely expressed, and the AKRP-GFP fusion localized to plastids of both gametophytes, in isolated chloroplast and co-localized with a plastid marker in pollen and pollen tubes. Cell-type-specific complementation of akrp-3 hinted at the developmental timing at which AKRP might play an essential role. Our findings provide a plausible insight into the crucial role of AKRP in the differentiation of both gametophytes and coupling embryo development with chlorophyll synthesis. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2023 Electronic address https://doi.org/10.3389/fpls.2022.767339
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