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Biological autoluminescence as a non-invasive monitoring tool for pulsed electric field effects on yeast cells
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SYSNO ASEP 0560868 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Biological autoluminescence as a non-invasive monitoring tool for pulsed electric field effects on yeast cells Author(s) Bereta, M. (SK)
Teplan, M. (SK)
Chafai, Djamel Eddine (FZU-D)
Cifra, Michal (URE-Y) RID, ORCID, SAINumber of authors 4 Article number 9232161 Source Title 33rd General Assembly and Scientific Symposium (GASS) of the International-Union-of-Radio-Science (URSI) 2020. - New York : IEEE, 2020 - ISBN 978-9-4639-6800-3 Number of pages 3 s. Publication form Online - E Action General Assembly and Scientific Symposium (GASS) of the International-Union-of-Radio-Science (URSI) /33./ Event date 29.08.2020 - 05.08.2020 VEvent location Řím Country IT - Italy Event type WRD Language eng - English Country US - United States Keywords biology ; electric fields ; monitoring ; biomedical measurement ; photonics ; tools ; pulse measure Subject RIV BO - Biophysics OECD category Medical laboratory technology (including laboratory samples analysis Subject RIV - cooperation Institute of Radio Engineering and Electronics - Electronics ; Optoelectronics, Electrical Engineering R&D Projects GA18-23597S GA ČR - Czech Science Foundation (CSF) Institutional support FZU-D - RVO:68378271 ; URE-Y - RVO:67985882 UT WOS 000632276400087 EID SCOPUS 85096869533 DOI 10.23919/URSIGASS49373.2020.9232161 Annotation The application of pulsed electric field (PEF) is nowadays becoming a very promising tool for application in medicine or food industry. However, the mechanisms of PEF interaction with living matter are still not fully elucidated. The aim of the presented work is to show the potential application of biological autoluminescence (BAL) for monitoring of pulsed electric field (PEF) biological effects on yeasts. The yeast cell culture (Saccharomyces cerevisiae) is exposed to the pulsed electric field (pulse duration: 0.1-5 ms, pulse number: 1-10, electric field strength: 3 kV.cm(-1)) and the BAL is monitored during exposure. The results indicate detectable differences in BAL dynamics when PEF is applied. Due to its non-invasive and label-free application, the BAL could be used as a beneficial tool for monitoring of PEF biological effects. Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2023 Electronic address https://ieeexplore.ieee.org/document/9232161/authors#authors
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