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The Effect of Sulforaphane on Perinatal Hypoxic-Ischemic Brain Injury in Rats
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SYSNO ASEP 0560055 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Effect of Sulforaphane on Perinatal Hypoxic-Ischemic Brain Injury in Rats Author(s) Kapoor, Sonam (FGU-C) ORCID
Kala, David (FGU-C)
Svoboda, Jan (FGU-C)
Daněk, Jan (FGU-C)
Faridová, Adéla (FGU-C) ORCID
Brnoliaková, Z. (SK)
Mikulecká, Anna (FGU-C) RID, ORCID
Folbergrová, Jaroslava (FGU-C) RID
Otáhal, Jakub (FGU-C) RID, ORCID, SAINumber of authors 9 Source Title Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 71, č. 3 (2022), s. 401-411Number of pages 11 s. Language eng - English Country CZ - Czech Republic Keywords perinatal hypoxic-ischemic insult ; rat ; FDG-PET ; sulforaphane ; neuronal damage ; motor impairment OECD category Neurosciences (including psychophysiology R&D Projects GA18-07908S GA ČR - Czech Science Foundation (CSF) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA22-28265S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000834626200006 EID SCOPUS 85135419620 DOI 10.33549/physiolres.934878 Annotation Perinatal hypoxic-ischemic insult (HII) is one of the main devastating causes of morbidity and mortality in newborns. HII induces brain injury which evolves to neurological sequelae later in life. Hypothermia is the only therapeutic approach available capable of diminishing brain impairment after HII. Finding a novel therapeutic method to reduce the severity of brain injury and its consequences is critical in neonatology. The present paper aimed to evaluate the effect of sulforaphane (SFN) pre-treatment on glucose metabolism, neurodegeneration, and functional outcome at the acute, sub-acute, and sub-chronic time intervals in the experimental model of perinatal hypoxic-ischemic insult in rats. To estimate the effect of SFN on brain glucose uptake we have performed 18F-deoxyglucose (FDG) ??CT/PET. The activity of FDG was determined in the hippocampus and sensorimotor cortex. Neurodegeneration was assessed by histological analysis of Nissl-stained brain sections. To investigate functional outcomes a battery of behavioral tests was employed. We have shown that although SFN possesses a protective effect on glucose uptake in the ischemic hippocampus 24 h and 1 week after HII, no effect has been observed in the motor cortex. We have further shown that the ischemic hippocampal formation tends to be thinner in HIE and SFN treatment tends to reverse this pattern. We have observed subtle chronic movement deficit after HII detected by ladder rung walking test with no protective effect of SFN. SFN should be thus considered as a potent neuroprotective drug with the capability to interfere with pathophysiological processes triggered by perinatal hypoxic-ischemic insult. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2023 Electronic address https://www.biomed.cas.cz/physiolres/pdf/2022/71_401.pdf
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