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In Vivo Contrast Imaging of Rat Heart with Carbon Dioxide Foam
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SYSNO ASEP 0559871 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title In Vivo Contrast Imaging of Rat Heart with Carbon Dioxide Foam Author(s) Karalko, A. (CZ)
Keša, P. (CZ)
Jelínek, F. (CZ)
Šefc, L. (CZ)
Ježek, Jan (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Grus, T. (CZ)Number of authors 7 Article number 5124 Source Title Sensors. - : MDPI
Roč. 22, č. 14 (2022)Number of pages 11 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords carbon dioxide foam ; angiography ; contrast-enhanced ultrasound imaging ; preclinical imaging Subject RIV BO - Biophysics OECD category Biophysics R&D Projects EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure Czech-BioImaging - 90062 - Ústav molekulární genetiky AV ČR, v. v. i. Method of publishing Open access Institutional support UPT-D - RVO:68081731 UT WOS 000832426000001 EID SCOPUS 85133518791 DOI 10.3390/s22145124 Annotation Widely used classical angiography with the use of iodine contrast agents is highly problematic, particularly in patients with diabetes mellitus, cardiac and pulmonary diseases, or degree III or IV renal insufficiency. Some patients may be susceptible to allergic reaction to the iodine contrast substance. The intravenous injection of a bolus of CO2 (negative contrast) is an alternative method, which is, however, currently only used for imaging blood vessels of the lower limbs. The aim of our project was to design and test on an animal model a methodology for injecting the CO2 foam which would minimize the possibility of embolization of the brain tissue and heart infarction, leading to their damage. This is important research for the further promotion of the use of CO2, which is increasingly important for endovascular diagnosis and treatment, because carbon-dioxide-related complications are extremely rare. CO2 foam was prepared by the rapid mixing in a 2:1 ratio of CO2 and fetal bovine serum (FBS)-enriched Dulbecco's Modified Eagle Medium (DMEM). Freshly prepared CO2 foam was administered into the catheterized rat tail vein or cannulated rat abdominal aorta and inferior vena cava (IVC). CO2 foam was compared with commercially available microbubbles (lipid shell/gas core). The rat heart in its parasternal long axis was imaged in B-Mode and Non-linear Contrast Mode before/during and after the contrast administration. Samples of the brain, heart and lungs were collected and subjected to histological examination. The non-linear contrast imaging method enables the imaging of micron-sized gas microbubbles inside a rat heart. The significantly shorter lifetime of the prepared CO2 foam is a benefit for avoiding the local ischemia of tissues. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2023 Electronic address https://www.mdpi.com/1424-8220/22/14/5124
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