Hypothermic Storage of 3D Cultured Multipotent Mesenchymal Stromal Cells for Regenerative Medicine Applications

Vacková, Irena; Vavřínová, E.; Musílková, Jana; Havlas, V.; Petrenko, Yuriy

doi:https://dx.doi.org/10.3390/polym14132553

Number of the records: 1

Hypothermic Storage of 3D Cultured Multipotent Mesenchymal Stromal Cells for Regenerative Medicine Applications

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SYSNO ASEP	0559406
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Hypothermic Storage of 3D Cultured Multipotent Mesenchymal Stromal Cells for Regenerative Medicine Applications
Author(s)	Vacková, Irena (FGU-C)_{ORCID, RID} Vavřínová, E. (CZ) Musílková, Jana (FGU-C)_{RID, ORCID} Havlas, V. (CZ) Petrenko, Yuriy (FGU-C)_{ORCID, RID, SAI}
Article number	2553
Source Title	Polymers. - : MDPI Roč. 14, č. 13 (2022)
Number of pages	13 s.
Language	eng - English
Country	CH - Switzerland
Keywords	multipotent mesenchymal stromal cells ; Wharton's jelly ; Hyalofast (R) ; hypothermic storage ; paracrine activity
OECD category	Biomaterials (as related to medical implants, devices, sensors)
R&D Projects	NV19-06-00355 GA MZd - Ministry of Health (MZ)
	LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823
UT WOS	000824092200001
EID SCOPUS	85132991122
DOI	10.3390/polym14132553
Annotation	The regulatory requirements in cell processing, in the choice of a biomaterial scaffold and in quality control analysis, have to be followed in the clinical application of tissue-engineered grafts. Confirmation of sterility during quality control studies requires prolonged storage of the cell-based construct. After storage, preservation of the functional properties of the cells is an important prerequisite if the cells are to be used for cell-based tissue therapies. The study presented here shows the generation of 3D constructs based on Wharton's jelly multipotent mesenchymal stromal cells (WJ-MSCs) and the clinically-acceptable HyaloFast (R) scaffold, and the effect of two- and six-day hypothermic storage of 3D cell-based constructs on the functional properties of populated cells. To study the viability, growth, gene expression, and paracrine secretion of WJ-MSCs within the scaffolds before and after storage, xeno-free culture conditions, metabolic, qPCR, and multiplex assays were applied. The WJ-MSCs adhered and proliferated within the 3D HyaloFast (R). Our results show different viability of the cells after the 3D constructs have been stored under mild (25 degrees C) or strong (4 degrees C) hypothermia. At 4 degrees C, the significant decrease of metabolic activity of WJ-MSCs was detected after 2 days of storage, with almost complete cell loss after 6 days. In mild hypothermia (25 degrees C) the decrease in metabolic activity was less remarkable, confirming the suitability of these conditions for cell preservation in 3D environment. The significant changes were detected in gene expression and in the paracrine secretion profile after 2 and 6 days of storage at 25 degrees C. The results presented in this study are important for the rapid transfer of tissue engineering approaches into clinical applications.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2023
Electronic address	https://www.mdpi.com/2073-4360/14/13/2553

Number of the records: 1