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Biomimetic biphasic curdlan-based scaffold for osteochondral tissue engineering applications Characterization and preliminary evaluation of mesenchymal stem cell response in vitro
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SYSNO ASEP 0558807 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biomimetic biphasic curdlan-based scaffold for osteochondral tissue engineering applications Characterization and preliminary evaluation of mesenchymal stem cell response in vitro Author(s) Klimek, K. (PL)
Benko, A. (PL)
Vandrovcová, Marta (FGU-C) RID, ORCID
Trávníčková, Martina (FGU-C) RID, ORCID, SAI
Douglas, T. E. L. (GB)
Tarczynska, M. (PL)
Brož, Antonín (FGU-C) RID, ORCID, SAI
Gaweda, K. (PL)
Ginalska, G. (PL)
Bačáková, Lucie (FGU-C) RID, ORCIDArticle number 212724 Source Title Biomaterials Advances. - : Elsevier
Roč. 135, April (2022)Number of pages 19 s. Language eng - English Country NL - Netherlands Keywords beta-1,3-Glucan ; biphasic scaffold ; osteochondral defects ; regenerative medicine ; stem cells ; tissue engineering OECD category Biomaterials (as related to medical implants, devices, sensors) R&D Projects NU20-08-00208 GA MZd - Ministry of Health (MZ) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF18_046/0016045 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support FGU-C - RVO:67985823 UT WOS 000812231700001 EID SCOPUS 85131954631 DOI 10.1016/j.bioadv.2022.212724 Annotation Osteochondral defects remain a huge problem in medicine today. Biomimetic bi- or multi-phasic scaffolds constitute a very promising alternative to osteochondral autografts and allografts. In this study, a new curdlanbased scaffold was designed for osteochondral tissue engineering applications. To achieve biomimetic properties, it was enriched with a protein component ??? whey protein isolate as well as a ceramic ingredient ??? hydroxyapatite granules. The scaffold was fabricated via a simple and cost-efficient method, which represents a significant advantage. Importantly, this technique allowed generation of a scaffold with two distinct, but integrated phases. Scanning electron microcopy and optical profilometry observations demonstrated that phases of biomaterial possessed different structural properties. The top layer of the biomaterial (mimicking the cartilage) was smoother than the bottom one (mimicking the subchondral bone), which is beneficial from a biological point of view because unlike bone, cartilage is a smooth tissue. Moreover, mechanical testing showed that the top layer of the biomaterial had mechanical properties close to those of natural cartilage. Although the mechanical properties of the bottom layer of scaffold were lower than those of the subchondral bone, it was still higher than in many analogous systems. Most importantly, cell culture experiments indicated that the biomaterial possessed high cytocompatibility towards adipose tissue-derived mesenchymal stem cells and bone marrow-derived mesenchymal stem cells in vitro. Both phases of the scaffold enhanced cell adhesion, proliferation, and chondrogenic differentiation of stem cells (revealing its chondroinductive properties in vitro) as well as osteogenic differentiation of these cells (revealing its osteoinductive properties in vitro). Given all features of the novel curdlan-based scaffold, it is worth noting that it may be considered as promising candidate for osteochondral tissue engineering applications. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2023 Electronic address https://doi.org/10.1016/j.bioadv.2022.212724
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