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Engineered Delivery of Dental Stem-Cell-Derived Extracellular Vesicles for Periodontal Tissue Regeneration
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SYSNO ASEP 0558806 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Engineered Delivery of Dental Stem-Cell-Derived Extracellular Vesicles for Periodontal Tissue Regeneration Author(s) Zárubová, Jana (FGU-C) RID, ORCID
Hasani-Sadrabadi, M. M. (US)
Dashtimoghadam, E. (US)
Zhang, X. (US)
Ansari, S. (US)
Li, S. (US)
Moshaverinia, A. (US)Number of authors 7 Article number 2102593 Source Title Advanced Healthcare Materials. - : Wiley - ISSN 2192-2640
Roč. 11, č. 12 (2022)Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords extracellular vesicles ; immunoengineering ; local drug delivery ; periodontal tissue healing ; periodontitis OECD category Dentistry, oral surgery and medicine Method of publishing Limited access Institutional support FGU-C - RVO:67985823 UT WOS 000764955700001 EID SCOPUS 85125564359 DOI 10.1002/adhm.202102593 Annotation Periodontal disease begins as an inflammatory response to a bacterial biofilm deposited around the teeth, which over time leads to the destruction of tooth-supporting structures and consequently tooth loss. Conventional treatment strategies show limited efficacy in promoting regeneration of damaged periodontal tissues. Here, a delivery platform is developed for small extracellular vesicles (sEVs) derived from gingival mesenchymal stem cells (GMSCs) to treat periodontitis. EVs can achieve comparable therapeutic effects to their cells of origin. However, the short half-lives of EVs after their administration along with their rapid diffusion away from the delivery site necessitate frequent administration to achieve therapeutic benefits. To address these issues, “dual delivery” microparticles are engineered enabling microenvironment-sensitive release of EVs by metalloproteinases at the affected site along with antibiotics to suppress bacterial biofilm growth. GMSC sEVs are able to decrease the secretion of pro-inflammatory cytokines by monocytes/macrophages and T cells, suppress T-cell activation, and induce the formation of T regulatory cells (Tregs) in vitro and in a rat model of periodontal disease. One-time administration of immunomodulatory GMSC sEV-decorated microparticles leads to a significant improvement in regeneration of the damaged periodontal tissue. This approach will have potential clinical applications in the regeneration of a variety of tissues. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2023 Electronic address https://doi.org/10.1002/adhm.202102593
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