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Derivatization in Analytical Chemistry
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SYSNO ASEP 0558090 Document Type M - Monograph Chapter R&D Document Type The record was not marked in the RIV Title Structural Characterization of Unusual Fatty Acid Methyl Esters with Double and Triple Bonds Using HPLC/APCI-MS2 with Acetonitrile In-Source Derivatization Author(s) Horká, Petra (UOCHB-X)
Vrkoslav, Vladimír (UOCHB-X) RID, ORCID
Kindl, Jiří (UOCHB-X) RID
Schwarzová-Pecková, K. (CZ)
Cvačka, Josef (UOCHB-X) RID, ORCIDSource Title Derivatization in Analytical Chemistry. - Basel : MDPI, 2022 / Tzanavaras P. D. - ISBN 978-3-0365-4256-0 Pages s. 39-59 Number of pages 21 s. Number of pages 200 Publication form Print - P Language eng - English Country CH - Switzerland Keywords acetonitrile-related adducts ; acetylenic lipids ; double and triple bond localization ; in-source derivatization ; mass spectrometry OECD category Analytical chemistry R&D Projects EF16_019/0000729 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UOCHB-X - RVO:61388963 DOI 10.3390/books978-3-0365-4255-3 Annotation Double and triple bonds have significant effects on the biological activities of lipids. Determining multiple bond positions in their molecules by mass spectrometry usually requires chemical derivatization. This work presents an HPLC/MS method for pinpointing the double and triple bonds in fatty acids. Fatty acid methyl esters were separated by reversed-phase HPLC with an acetonitrile mobile phase. In the APCI source, acetonitrile formed reactive species, which added to double and triple bonds to form [M + C3H5N]+• ions. Their collisional activation in an ion trap provided fragments helpful in localizing the multiple bond positions. This approach was applied to fatty acids with isolated, cumulated, and conjugated double bonds and triple bonds. The fatty acids were isolated from the fat body of early-nesting bumblebee Bombus pratorum and seeds or seed oils of Punicum granatum, Marrubium vulgare, and Santalum album. Using the method, the presence of the known fatty acids was confirmed, and new ones were discovered. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2023
Number of the records: 1