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Nicotinic Acetylcholine Receptors Expressed by Striatal Interneurons Inhibit Striatal Activity and Control Striatal-Dependent Behaviors

SYSNO ASEP	0557064
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Nicotinic Acetylcholine Receptors Expressed by Striatal Interneurons Inhibit Striatal Activity and Control Striatal-Dependent Behaviors
Author(s)	Abbondanza, Alice (FGU-C)_ORCID Ribeiro Bas, Irina (FGU-C)_ORCID Modrák, Martin (MBU-M)_ORCID Čapek, Martin (FGU-C)_{RID, ORCID} Minich, Jessica (FGU-C) Tyshkevich, Alexandra (FGU-C) Naser, Shahed (FGU-C) Rangotis, Revan (FGU-C)_ORCID Houdek, Pavel (FGU-C)_ORCID Sumová, Alena (FGU-C)_{RID, ORCID} Dumas, S. (FR) Bernard, V. (FR) Janíčková, Helena (FGU-C)_{RID, ORCID, SAI}
Number of authors	13
Source Title	Journal of Neuroscience. - : Society for Neuroscience - ISSN 0270-6474 Roč. 42, č. 13 (2022), s. 2786-2803
Number of pages	18 s.
Language	eng - English
Country	US - United States
Keywords	acetylcholine ; behavior ; Fos ; interneurons ; nAChRs ; striatum
OECD category	Neurosciences (including psychophysiology
R&D Projects	GJ19-07983Y GA ČR - Czech Science Foundation (CSF)
	LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	8J21FR022 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2018131 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF18_046/0016045 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823 ; MBU-M - RVO:61388971
UT WOS	000779618200003
EID SCOPUS	85128160080
DOI	10.1523/JNEUROSCI.1627-21.2022
Annotation	Acetylcholine is an important modulator of striatal activity, and it is vital to controlling striatal-dependent behaviors, including motor and cognitive functions. Despite this significance, the mechanisms determining how acetylcholine impacts striatal signaling are still not fully understood. In particular, little is known about the role of nAChRs expressed by striatal interneurons. In the present study, we used FISH to determine which neuronal types express the most prevalent beta2 nicotinic subunit in the mouse striatum. Our data support a common view that nAChR expression is mostly restricted to striatal interneurons. Surprisingly though, cholinergic interneurons were identified as a population with the highest expression of beta2 nicotinic subunit. To investigate the functional significance of beta2containing nAChRs in striatal interneurons, we deleted them by injecting the AAV-Cre vector into the striatum of beta2-flox/flox male mice. The deletion led to alterations in several behavioral domains, namely, to an increased anxiety-like behavior, decrease in sociability ratio, deficit in discrimination learning, and increased amphetamine-induced hyperlocomotion and c-Fos expression in mice with beta2 deletion. Further colocalization analysis showed that the increased c-Fos expression was present in both medium spiny neurons and presumed striatal interneurons. The present study concludes that, despite being relatively rare, beta2-containing nAChRs are primarily expressed in striatal neurons by cholinergic interneurons and play a significant role in behavior.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2023
Electronic address	https://doi.org/10.1523/JNEUROSCI.1627-21.2022

Number of the records: 1