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Culture Isolate of Rickettsia felis from a Tick
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SYSNO ASEP 0556827 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Culture Isolate of Rickettsia felis from a Tick Author(s) Danchenko, M. (SK)
Benada, Oldřich (MBU-M) ORCID, RID
Škultéty, L'udovít (MBU-M) ORCID, RID
Sekeyova, Z. (CZ)Number of authors 4 Article number 4321 Source Title International Journal of Environmental Research and Public Health. - : MDPI - ISSN 1661-7827
Roč. 19, č. 7 (2022)Number of pages 18 s. Language eng - English Country CH - Switzerland Keywords flea-borne rickettsioses ; fever group rickettsiae ; real-time pcr ; spotted-fever ; ixodes-ricinus ; phylogenetic analysis ; molecular-detection ; genetic diversity ; genus rickettsia ; cat fleas ; Rickettsia felis ; vector-borne bacteria ; Ixodes ricinus ; shell-vial technique ; cell culture Subject RIV EE - Microbiology, Virology OECD category Microbiology Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000781874400001 EID SCOPUS 85127608511 DOI 10.3390/ijerph19074321 Annotation Although the cat flea, Ctenocephalides felis, has been identified as the primary vector of Rickettsia felis, additional flea, tick, mite, and louse species have also been associated with this bacterium by molecular means, however, the role of these arthropods in the transmission of R. felis has not been clarified. Here, we succeeded in culture isolation of R. felis from a host-seeking castor bean tick, Ixodes ricinus, the most common tick in Slovakia. The bacterial isolation was performed on XTC-2 cells at 28 degrees C using the shell-vial technique. An evaluation of the growth properties was performed for both the XTC-2 and Vero cell lines. We observed R. felis in the infected host cells microscopically by Gimenez staining and immunofluorescence assay. The R. felis isolate was purified by gradient ultracentrifugation and visualized by electron microscopy. Fragments of the genes gltA, ompA, ompB, htrA, rpoB, sca4, rffE, and rrs were amplified and compared with the corresponding sequences of the type strain URRWXCal2 and other R. felis cultureisolated strains. We did not detect any nucleotide polymorphisms, however, plasmid pRF delta, characteristic of the standard strain, was absent in our isolate. Herein, we describe the first successful isolation and characterization of a tick-derived R. felis strain ´Danube´, obtained from an I. ricinus nymph. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://www.mdpi.com/1660-4601/19/7/4321
Number of the records: 1