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Coating Ti6Al4V implants with nanocrystalline diamond functionalized with BMP-7 promotes extracellular matrix mineralization in vitro and faster osseointegration in vivo
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SYSNO ASEP 0556620 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Coating Ti6Al4V implants with nanocrystalline diamond functionalized with BMP-7 promotes extracellular matrix mineralization in vitro and faster osseointegration in vivo Author(s) Němčáková, Ivana (FGU-C) RID, ORCID
Litvinec, Andrej (FGU-C)
Mandys, V. (CZ)
Potocký, Štěpán (FZU-D) RID, ORCID
Plencner, Martin (FGU-C) RID, ORCID
Doubková, Martina (FGU-C) ORCID, RID
Naňka, O. (CZ)
Olejníčková, Veronika (FGU-C) RID, ORCID, SAI
Šaňková, Barbora (FGU-C) RID, ORCID
Bartoš, M. (CZ)
Ukraintsev, E. (CZ)
Babčenko, O. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCID
Kromka, Alexander (FZU-D) RID, ORCID, SAI
Rezek, B. (CZ)
Sedmera, David (FGU-C) RID, ORCID, SAINumber of authors 16 Article number 5264 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 12, č. 1 (2022)Number of pages 17 s. Language eng - English Country GB - United Kingdom Keywords alkaline-phosphatase activity ; human osteogenic protein-1 ; mesenchymal stem-cells ; bone-formation ; surface functionalization ; antiadhesive properties ; metallic implants ; titanium implants ; dental implants OECD category Biomaterials (as related to medical implants, devices, sensors) Subject RIV - cooperation Institute of Physics - Surgery incl. Transplants R&D Projects GA21-06065S GA ČR - Czech Science Foundation (CSF) NV15-32497A GA MZd - Ministry of Health (MZ) LM2018110 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_019/0000760 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure CzechNanoLab - 90110 - Vysoké učení technické v Brně Method of publishing Open access Institutional support FGU-C - RVO:67985823 ; FZU-D - RVO:68378271 UT WOS 000774204500044 EID SCOPUS 85127270573 DOI https://doi.org/10.1038/s41598-022-09183-z Annotation The present study investigates the effect of an oxidized nanocrystalline diamond (O-NCD) coating functionalized with bone morphogenetic protein 7 (BMP-7) on human osteoblast maturation and extracellular matrix mineralization in vitro and on new bone formation in vivo. The chemical structure and the morphology of the NCD coating and the adhesion, thickness and morphology of the superimposed BMP-7 layer have also been assessed. The material analysis proved synthesis of a conformal diamond coating with a fine nanostructured morphology on the Ti6Al4V samples. The homogeneous nanostructured layer of BMP-7 on the NCD coating created by a physisorption method was confirmed by AFM. The osteogenic maturation of hFOB 1.19 cells in vitro was only slightly enhanced by the O-NCD coating alone without any increase in the mineralization of the matrix. Functionalization of the coating with BMP-7 resulted in more pronounced cell osteogenic maturation and increased extracellular matrix mineralization. Similar results were obtained in vivo from micro-CT and histological analyses of rabbit distal femurs with screws implanted for 4 or 12 weeks. While the O-NCD-coated implants alone promoted greater thickness of newly-formed bone in direct contact with the implant surface than the bare material, a further increase was induced by BMP-7. It can be therefore concluded that O-NCD coating functionalized with BMP-7 is a promising surface modification of metallic bone implants in order to improve their osseointegration. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2023 Electronic address https://doi.org/10.1038/s41598-022-09183-z
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