Number of the records: 1
Structural Characterization of Mono- and Dimethylphosphatidylethanolamines from Various Organisms Using a Complex Analytical Strategy including Chiral Chromatography
- 1.
SYSNO ASEP 0556540 Document Type J - Journal Article R&D Document Type The record was not marked in the RIV Subsidiary J Článek ve WOS Title Structural Characterization of Mono- and Dimethylphosphatidylethanolamines from Various Organisms Using a Complex Analytical Strategy including Chiral Chromatography Author(s) Řezanka, Tomáš (MBU-M) ORCID
Palyzová, Andrea (MBU-M) RID, ORCID
Vítová, Milada (MBU-M) RID, ORCID
Brányik, T. (CZ)
Kulišová, M. (CZ)
Jarošová Kolouchová, I. (CZ)Article number 616 Source Title Symmetry-Basel. - : MDPI
Roč. 14, č. 3 (2022)Number of pages 20 s. Language eng - English Country CH - Switzerland Keywords performance liquid-chromatography ; tandem mass-spectrometry ; chain fatty-acids ; phosphatidylcholine biosynthesis ; hydrophilic interaction ; shotgun lipidomics ; multiple precursor ; phosphatidic-acid ; polar lipids ; esi-ms/ms ; mono- and dimethylphosphatidylethanolamines ; bacteria ; fungi ; animals ; algae ; chiral chromatography Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000774310100001 EID SCOPUS 85127355417 DOI 10.3390/sym14030616 Annotation Two minor phospholipids, i.e., mono- and/or dimethylphosphatidylethanolamines, are widespread in many organisms, from bacteria to higher plants and animals. A molecular mixture of methyl-PE and dimethyl-PE was obtained from total lipids by liquid chromatography and further identified by mass spectrometry. Total methyl-PE and dimethyl-PE were cleaved by phospholipase C, and the resulting diacylglycerols, in the form of acetyl derivatives, were separated into alkyl-acyl, alkenyl-acyl, and diacylglycerols. Reversed-phase LC/MS allowed dozens of molecular species to be identified and further analyzed. This was performed on a chiral column, and identification by tandem positive ESI revealed that diacyl derivatives from all four bacteria were mixtures of both R and S enantiomers. The same applied to alkenyl-acyl derivatives of anaerobic bacteria. Analysis thus confirmed that some bacteria biosynthesize phospholipids having both sn-glycerol-3-phosphate and sn-glycerol-1-phosphate as precursors. These findings were further supported by data already published in GenBank. The use of chiral chromatography made it possible to prove that both enantiomers of glycerol phosphate of some molecular species of mono- and dimethylphosphatidylethanolamines are present. The result of the analysis can be interpreted that the cultured bacteria do not have homochiral membranes but, on the contrary, have an asymmetric, i.e., heterochiral membranes. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://www.mdpi.com/2073-8994/14/3/616
Number of the records: 1