Number of the records: 1
DIACYLGLYCEROL KINASE 5 regulates polar tip growth of tobacco pollen tubes
- 1.
SYSNO ASEP 0556437 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title DIACYLGLYCEROL KINASE 5 regulates polar tip growth of tobacco pollen tubes Author(s) Scholz, P. (DE)
Pejchar, Přemysl (UEB-Q) RID, ORCID
Fernkorn, M. (DE)
Škrabálková, Eliška (UEB-Q) ORCID
Pleskot, Roman (UEB-Q) RID, ORCID
Blersch, K. (DE)
Munnik, T. (NL)
Potocký, Martin (UEB-Q) RID, ORCID
Ischebeck, T. (DE)Number of authors 9 Source Title New Phytologist - ISSN 0028-646X
Roč. 233, č. 5 (2022), s. 2185-2202Number of pages 18 s. Language eng - English Country GB - United Kingdom Keywords diacylglycerol kinase ; lipid signaling ; pectin OECD category Cell biology R&D Projects GA17-27477S GA ČR - Czech Science Foundation (CSF) GA20-21547S GA ČR - Czech Science Foundation (CSF) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000740760700001 EID SCOPUS 85122680384 DOI 10.1111/nph.17930 Annotation Pollen tubes require a tightly regulated pectin secretion machinery to sustain the cell wall plasticity required for polar tip growth. Involved in this regulation at the apical plasma membrane are proteins and signaling molecules, including phosphoinositides and phosphatidic acid (PA). However, the contribution of diacylglycerol kinases (DGKs) is not clear. We transiently expressed tobacco DGKs in pollen tubes to identify a plasma membrane (PM)-localized isoform, and then to study its effect on pollen tube growth, pectin secretion and lipid signaling. In order to potentially downregulate DGK5 function, we overexpressed an inactive variant. Only one of eight DGKs displayed a confined localization at the apical PM. We could demonstrate its enzymatic activity and that a kinase-dead variant was inactive. Overexpression of either variant led to differential perturbations including misregulation of pectin secretion. One mode of regulation could be that DGK5-formed PA regulates phosphatidylinositol 4-phosphate 5-kinases, as overexpression of the inactive DGK5 variant not only led to a reduction of PA but also of phosphatidylinositol 4,5-bisphosphate levels and suppressed related growth phenotypes. We conclude that DGK5 is an additional player of polar tip growth that regulates pectin secretion probably in a common pathway with PI4P 5-kinases. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2023 Electronic address http://doi.org/10.1111/nph.17930
Number of the records: 1