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Karyotype Differentiation in Cultivated Chickpea Revealed by Oligopainting Fluorescence in situ Hybridization

SYSNO ASEP	0556433
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Karyotype Differentiation in Cultivated Chickpea Revealed by Oligopainting Fluorescence in situ Hybridization
Author(s)	Doležalová, Alžběta (UEB-Q)_ORCID Sládeková, Lucia (UEB-Q) Šimoníková, Denisa (UEB-Q)_ORCID Holušová, Kateřina (UEB-Q)_ORCID Karafiátová, Miroslava (UEB-Q)_{RID, ORCID} Varshney, R.K. (IN) Doležel, Jaroslav (UEB-Q)_{RID, ORCID} Hřibová, Eva (UEB-Q)_{RID, ORCID}
Number of authors	8
Article number	791303
Source Title	Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X Roč. 12, JAN 25 (2022)
Number of pages	11 s.
Language	eng - English
Country	CH - Switzerland
Keywords	chromosome identification ; chromosome translocation ; Cicer arietinumL ; desi type ; kabuli type ; oligopainting FISH
OECD category	Biochemistry and molecular biology
R&D Projects	EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UEB-Q - RVO:61389030
UT WOS	000752622200001
EID SCOPUS	85124258869
DOI	10.3389/fpls.2021.791303
Annotation	Chickpea (Cicer arietinum L.) is one of the main sources of plant proteins in the Indian subcontinent and West Asia, where two different morphotypes, desi and kabuli, are grown. Despite the progress in genome mapping and sequencing, the knowledge of the chickpea genome at the chromosomal level, including the long-range molecular chromosome organization, is limited. Earlier cytogenetic studies in chickpea suffered from a limited number of cytogenetic landmarks and did not permit to identify individual chromosomes in the metaphase spreads or to anchor pseudomolecules to chromosomes in situ. In this study, we developed a system for fast molecular karyotyping for both morphotypes of cultivated chickpea. We demonstrate that even draft genome sequences are adequate to develop oligo-fluorescence in situ hybridization (FISH) barcodes for the identification of chromosomes and comparative analysis among closely related chickpea genotypes. Our results show the potential of oligo-FISH barcoding for the identification of structural changes in chromosomes, which accompanied genome diversification among chickpea cultivars. Moreover, oligo-FISH barcoding in chickpea pointed out some problematic, most probably wrongly assembled regions of the pseudomolecules of both kabuli and desi reference genomes. Thus, oligo-FISH appears as a powerful tool not only for comparative karyotyping but also for the validation of genome assemblies.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2023
Electronic address	http://doi.org/10.3389/fpls.2021.791303

Number of the records: 1