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Adenosine Kinase Isoforms in the Developing Rat Hippocampus after LiCl/Pilocarpine Status Epilepticus

SYSNO ASEP	0556130
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Adenosine Kinase Isoforms in the Developing Rat Hippocampus after LiCl/Pilocarpine Status Epilepticus
Author(s)	Fábera, Petr (FGU-C)_ORCID Uttl, Libor (FGU-C)_ORCID Kubová, Hana (FGU-C)_{RID, ORCID} Tsenov, Grygoriy (FGU-C)_{RID, ORCID} Mareš, Pavel (FGU-C)_{RID, ORCID}
Number of authors	5
Article number	2510
Source Title	International Journal of Molecular Sciences. - : MDPI Roč. 23, č. 5 (2022)
Number of pages	18 s.
Language	eng - English
Country	CH - Switzerland
Keywords	adenosine kinase ; isoforms ; inhibitor ; development ; hippocampus ; epileptic afterdischarges ; rat ; LiCl/pilocarpine ; status epilepticus
OECD category	Pharmacology and pharmacy
R&D Projects	GA19-11931S GA ČR - Czech Science Foundation (CSF)
	EF16_025/0007444 GA MZd - Ministry of Health (MZ)
	GA18-09296S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823
UT WOS	000771797800001
EID SCOPUS	85125060689
DOI	10.3390/ijms23052510
Annotation	LiCl/pilocarpine status epilepticus (SE) induced in immature rats leads, after a latent period, to hippocampal hyperexcitability. The excitability may be influenced by adenosine, which exhibits anticonvulsant activity. The concentration of adenosine is regulated by adenosine kinase (ADK) present in two isoforms-ADK-L and ADK-S. The main goal of the study is to elucidate the changes in ADK isoform expression after LiCl/pilocarpine SE and whether potential changes, as well as inhibition of ADK by 5-iodotubercidin (5-ITU), may contribute to changes in hippocampal excitability during brain development. LiCl/pilocarpine SE was elicited in 12-day-old rats. Hippocampal excitability in immature rats was studied by the model of hippocampal afterdischarges (ADs), in which we demonstrated the potential inhibitory effect of 5-ITU. ADs demonstrated significantly decreased hippocampal excitability 3 days after SE induction, whereas significant hyperexcitability after 20 days compared to controls was shown. 5-ITU administration showed its inhibitory effect on the ADs in 32-day-old SE rats compared to SE rats without 5-ITU. Moreover, both ADK isoforms were examined in the immature rat hippocampus. The ADK-L isoform demonstrated significantly decreased expression in 12-day-old SE rats compared to the appropriate naive rats, whereas increased ADK-S isoform expression was revealed. A decreasing ADK-L/-S ratio showed the declining dominance of ADK-L isoform during early brain development. LiCl/pilocarpine SE increased the excitability of the hippocampus 20 days after SE induction. The ADK inhibitor 5-ITU exhibited anticonvulsant activity at the same age. Age-related differences in hippocampal excitability after SE might correspond to the development of ADK isoform levels in the hippocampus.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2023
Electronic address	https://www.mdpi.com/1422-0067/23/5/2510

Number of the records: 1